| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-08-08 |
| タイトル |
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|
タイトル |
Development of a Novel, Highly Sensitive System for Evaluating Ebola Virus Particle Formation |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Ebola virus |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
VP40 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
HiBiT system |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
viral particle formation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
antiviral screening |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Furuyama, Wakako
Sakaguchi, Miako
Ariyoshi, Hanako
Nanbo, Asuka
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Ebola virus (EBOV) causes severe hemorrhagic fevers in humans, and effective countermeasures remain limited. The EBOV-encoded major matrix protein VP40 is essential for viral assembly, budding, and particle release, making it a promising target for antiviral drug development. However, no approved drugs currently target the viral particle formation process. In this study, we established a simple and highly sensitive screening system to evaluate VP40-mediated virus-like particle (VLP) formation under biosafety level −2 conditions. The system uses the HiBiT luminescence-based reporter fused to VP40, allowing for the detection of VP40 release. Our results demonstrate that the HiBiT sequence fused at the N-terminus [HiBiT-VP40 (N)] retains VP40′s ability to form VLPs, supporting its use as a functional reporter. Furthermore, we validated the system by assessing the role of Rab11-dependent trafficking in VP40-mediated budding and by evaluating the effect of nocodazole, a microtubule depolymerizer, on VLP release. This novel screening system provides a convenient and reliable platform for screening potential inhibitors targeting the late stages of EBOV infection, including viral particle formation and release. Additionally, its potential adaptability to other filoviruses suggests wide applicability in the discovery and development of additional novel therapeutic agents. |
|
言語 |
en |
| 書誌情報 |
en : Viruses
巻 17,
号 7,
p. art. no. 1016,
発行日 2025-07-19
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| 出版者 |
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|
出版者 |
MDPI |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1999-4915 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.3390/v17071016 |
| 権利 |
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|
権利情報 |
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Viruses, 17(7), art. no. 1016; 2025 |
|
言語 |
en |
V17_1016.pdf (2.3 MB)
