| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-08-26 |
| タイトル |
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タイトル |
Prevotella intermedia Synergistically Exacerbates Pneumonia Induced by Oral Streptococci |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
neutrophils |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
oral streptococci |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
pneumonia |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
Prevotella intermedia |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
type IX secretion system |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Ashizawa, Hiroki
Iwanaga, Naoki
Nemoto, Kazuki
Hirayama, Tatsuro
Yoshida, Masataka
Takeda, Kazuaki
Ide, Shotaro
Tashiro, Masato
Hosogaya, Naoki
Takazono, Takahiro
Kosai, Kosuke
Sakamoto, Noriho
Izumikawa, Koichi
Naito, Mariko
Tanaka, Yoshimasa
Yanagihara, Katsunori
Yatera, Kazuhiro
Mukae, Hiroshi
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Background: The precise mechanisms of respiratory infection caused by oral anaerobic bacteria remain elusive. Unexpectedly, bacterial microbiota analysis with 16S rRNA revealed “hidden” mixed infections of anaerobic bacteria and commensal oral Streptococcus species in patients with community-acquired pneumonia. The study aimed to elucidate the mechanisms by which Prevotella intermedia exacerbates oral streptococcal pneumonia. Methods: Oral streptococci were oropharyngeally administered with the culture supernatants of P intermedia (PiSup) in mice to assess survival, microbial burden, inflammatory responses, and host response via unbiased bulk RNA sequencing. Additionally, genetically engineered strains of P intermedia were used to identify pathogenic factors. Results: Seven-week-old female C57BL/6J mice treated with the combination of Streptococcus spp and PiSup exhibited significantly worse survival and increased microbial burden in the lungs and spleen when compared with Streptococcus spp and control medium. RNA sequencing of whole lung revealed disruption of neutrophilic bactericidal activity due to NADPH downregulation, accompanied by reduced myeloperoxidase production in mixed infections, leading to dysfunctional neutrophil accumulation in the lungs. Notably, PiSup from strains bearing mutations in the type IX secretion pathway (ΔporT or ΔporK) failed to worsen the mixed infection. Conclusions: P intermedia exacerbates pneumonia caused by commensal oral streptococci by inducing neutrophilic dysfunction in mixed infection. Products of the type IX secretion system should be investigated as novel targets independent of reported virulence factors. |
|
言語 |
en |
| 書誌情報 |
en : The Journal of Infectious Diseases
巻 232,
号 2,
p. e280-e289,
発行日 2025-05-27
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| 出版者 |
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出版者 |
Oxford University Press |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0022-1899 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1093/infdis/jiaf278 |
| 権利 |
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|
権利情報 |
© The Author(s) 2025. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com. |
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言語 |
en |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
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内容記述タイプ |
Other |
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内容記述 |
Journal of Infectious Diseases, 232(2), pp.e280-e289; 2025 |
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言語 |
en |
JID232_e280.pdf (1.2 MB)
