| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-09-09 |
| タイトル |
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|
タイトル |
Calorie restriction in radiation-exposed mice affects the expression of autophagy-related protein p62 |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Calorie restriction |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Carcinogenesis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Radiation |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Nakayama, Takafumi
Suzuki, Keiji
Mitsutake, Norisato
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Background: Calorie restriction (CR) is known to decrease not only spontaneous but also chemical- and radiation-induced carcinogenesis. However, the mechanism of anti-cancer effect by CR is extremely complex and not fully understood. In this study, we focused on changes in autophagy-related proteins (LC3 and p62) as factors related to changes in nutritional status and the suppression of carcinogenesis by CR. Method: B6C3F1 male mice were exposed to 0 Gy or 3.8 Gy X-rays at 1 week of age, and then both groups were fed either a standard diet (95 kcal/week) or a calorie-restricted diet with approximately 30% fewer calories (65 kcal/week) from 7 weeks of age. Mice were sacrificed at several time points, and their livers were collected and analyzed. Results: CR suppressed the development of liver tumors in the irradiated group during the study period. The expression level of LC3 did not change significantly with or without CR and irradiation. On the other hand, the expression of p62 was significantly and temporarily increased in the irradiated CR group, in which radiation carcinogenesis was suppressed. Using immunofluorescence analysis, we found cells expressed p62 strongly throughout the cytoplasm in the CR group. In some of the p62-strong positive cells, the disappearance of the cell nucleus and a decrease in the nuclear DNA content were observed, and it was not co-localized with the cell proliferation marker, suggesting that cell death had occurred. Conclusions: Long-term CR, which inhibits radiation carcinogenesis, did not significantly affect autophagy activity in the liver as determined by LC3-II levels. On the other hand, increased expression of p62 was observed in the CR group in which the anti-cancer effect was confirmed. In particular, p62 strongly positive cells with smaller or no nuclei were detected exclusively in the CR group, suggesting a novel p62-mediated mechanism in the suppression of radiation-induced liver carcinogenesis by CR. |
|
言語 |
en |
| 書誌情報 |
en : BMC Cancer
巻 25,
号 1,
p. art. no. 1388,
発行日 2025-08-28
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| 出版者 |
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出版者 |
BioMed Central Ltd |
|
言語 |
en |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1471-2407 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1186/s12885-025-14771-z |
| 権利 |
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|
権利情報 |
© The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/. |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
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内容記述タイプ |
Other |
|
内容記述 |
BMC Cancer, 25(1), art. no. 1388; 2025 |
|
言語 |
en |
BMCC25_1388.pdf (1.6 MB)
