| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-09-09 |
| タイトル |
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タイトル |
ZFP36L1 and L2 as novel antiviral factors for Crimean-Congo hemorrhagic fever virus via interaction with viral nucleoprotein |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
infectious disease |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
high-throughput screening (HTS) |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
inhibition mechanism |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
protein–protein interaction |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
RNA degradation |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
RNA virus |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
Crimean-Congo hemorrhagic fever virus |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
viral immunologyvirology |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Hirano, Minato
Nochi, Koki
Matsugi, Momoko
Furukawa, Chie
Takeda, Hiroyuki
Sakurai, Yasuteru
Kurosaki, Yohei
Sawasaki, Tatsuya
Yasuda, Jiro
Takahashi, Hirotaka
Yoshii, Kentaro
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Crimean-Congo hemorrhagic fever virus (CCHFV) belongs to the genus Orthonairovirus and is the causative agent of viral hemorrhagic fever with a case fatality rate of 30%. Like many other viral proteins, the nucleoprotein (N) interacts with host factors during viral replication, leading to both pro- and anti-viral consequences. However, few studies have explored protein-protein interactions (PPI) between N and host proteins. In this study, we screened the PPI with 1116 human transcription factors and regulators using the AlphaScreen assay, which employs a cell-free synthesized human protein library. The host RNA-binding proteins, ZFP36L1 and L2, were identified through this screening, and further functional analyses revealed that both proteins significantly inhibited CCHFV minigenome replication. N and ZFP36 proteins interacted within cells, and the expression of N altered the intracellular localization of ZFP36 proteins. Interestingly, the RNA-binding activity of ZFP36s was not essential for the interaction and inhibition of CCHFV minigenome replication. A reporter assay using TNFA and IFNG UTRs, target RNAs of ZFP36 proteins, showed that CCHFV N activated ZFP36-mediated mRNA degradation. Further analysis revealed that the N-terminal region of ZFP36L1 was important for its interaction with CCHFV N. Deletion of the N-terminus of ZFP36L1 decreased its inhibitory effect on the minigenome, but not on the TNFA and IFNG reporters, suggesting context-dependent regulation of RNA degradation. This study demonstrated the applicability of PPI screening using protein array and AlphaScreen technology for investigating viral-host interactions. Further studies will contribute to understanding the antiviral immunity driven by host proteins and the corresponding viral countermeasures. |
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言語 |
en |
| 書誌情報 |
en : Journal of Biological Chemistry
巻 301,
号 9,
p. art. no. 110545,
発行日 2025-09-01
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| 出版者 |
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出版者 |
Elsevier Inc |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
00219258 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.jbc.2025.110545 |
| 権利 |
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権利情報 |
© 2025 THE AUTHORS. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
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言語 |
en |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
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内容記述タイプ |
Other |
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内容記述 |
Journal of Biological Chemistry, 301(9), art. no. 110545; 2025 |
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言語 |
en |
JBC301_110545.pdf (6.1 MB)
