| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-10-07 |
| タイトル |
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|
タイトル |
Genes with altered expression by 5-Aza treatment in myeloid leukemia cells through methylation in intron 1 |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Long read sequence |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Azacitidine |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA methylation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Myeloid leukemia |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Intron 1 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Fujioka, Machiko
Mishima, Hiroyuki
Itonaga, Hidehiro
Hamaguchi, Yo
Korzun, Uladzislau
Ando, Koji
Kinoshita, Akira
Miyazaki, Yasushi
Yoshiura, Koh-ichiro
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
5-Azacitidine (5-Aza) is a hypomethylating agent with demonstrated therapeutic efficacy for myeloid leukemia. The aim of this study was to identify the genes that mediate the cell killing effect of 5-Aza against leukemia cells through their expression changes and DNA demethylation. RNA sequencing revealed 54 genes with increased transcription levels in both SKM-1 and KG-1a myeloid leukemia cell lines treated with 5-Aza. Long read sequencing revealed 79 genes in which intron 1 exhibited significant DNA demethylation after 5-Aza treatment. Forty-three genes showed both increased gene expression and DNA demethylation in intron 1 in cells treated with 5-Aza. Enrichment signaling pathway analysis demonstrated that genes were associated with “amino acid metabolism,” “neutrophil degranulation,” and “DNA damage response”. We evaluated the prognostic impacts of the 43 genes in acute myeloid leukemia patients using TCGA database. The results revealed that two genes (HDC and MICALL2) with increased expression in leukemia cells after 5-Aza treatment were associated with better overall survival, while six genes (BTG2, CD52, PECAM1, PIK3IP1, PTGS2, and TREML2) correlated with worse overall survival. This study revealed that the epigenetic regulation by DNA demethylation in intron 1 has an important role of 5-Aza treatment in myeloid leukemia cells, and suggests novel targets for the development of combination therapy with 5-Aza. |
|
言語 |
en |
| 書誌情報 |
en : Leukemia Research
巻 158,
p. art. no. 108112,
発行日 2025-09-27
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| 出版者 |
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出版者 |
Elsevier Ltd |
|
言語 |
en |
| ISSN |
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|
収録物識別子タイプ |
ISSN |
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収録物識別子 |
01452126 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.leukres.2025.108112 |
| 権利 |
|
|
権利情報 |
© 2025 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Leukemia Research, 158, art. no. 108112; 2025 |
|
言語 |
en |
LR158_108112.pdf (4.6 MB)
