| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-10-09 |
| タイトル |
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|
タイトル |
Utility of the Redox Cycle of Nitrofurantoin for the Development of a New Chemiluminescence Method for Its Analysis in Milk Samples |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
nitrofurantoin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
growth promoter |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
food quality control |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
redox cycle |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
luminol |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
chemiluminescence |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
milk samples |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
El-Maghrabey, Mahmoud
Abdel-Hakim, Ali
Tagaya, Shiho
Kuroda, Naotaka
Kishikawa, Naoya
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Nitrofurantoin is utilized in various industries, including dairy, livestock, poultry, and aquaculture, as a growth promoter and antibacterial agent. Because prolonged use can cause mutagenesis and other side effects, many countries have prohibited its use in food-producing animals. In this work, we introduce a simple, rapid, and highly sensitive chemiluminescence (CL) approach for the quantitation of nitrofurantoin using its redox cycle activity. Nitrofurantoin is reduced to nitrofurantoin radicals by the reducing agent dithiothreitol, and reactive oxygen species (ROS) formed during the reoxidation process (superoxide anion radical) are detected by luminol CL. The CL conditions were optimized, including types of solvents, CL and reducing reagents, and their concentrations. The method was validated as per International Council for Harmonization (ICHQ2(R2)) guidelines, regarding linearity, detection and quantitation limits, accuracy, and precision. A good linearity with r = 0.9992 was obtained between the CL intensity and the nitrofurantoin concentration in the range of 4.0–400.0 ng/mL with a high sensitivity down to 1.15 ng/mL. The method was utilized to determine nitrofurantoin in milk samples, and a good recovery range was obtained (97.5–103.1%; RSD ≤ 4.4%); the results were comparable to the reported method, demonstrating the method’s reliability. Finally, the method demonstrated good practicality using a recently developed assessment tool. |
|
言語 |
en |
| 書誌情報 |
en : Molecules
巻 30,
号 18,
p. art. no. 3698,
発行日 2025-09-11
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| 出版者 |
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出版者 |
MDPI |
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言語 |
en |
| ISSN |
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|
収録物識別子タイプ |
EISSN |
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収録物識別子 |
1420-3049 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.3390/molecules30183698 |
| 権利 |
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|
権利情報 |
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Molecules, 30(18), art. no. 3698; 2025 |
|
言語 |
en |
M30_3698.pdf (2.1 MB)
