| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-10-21 |
| タイトル |
|
|
タイトル |
Evaluating the Potential of Galactosaminogalactan as a Diagnostic Target for Invasive Aspergillosis |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Aspergillus flavus |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Aspergillus fumigatus |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
chronic pulmonary aspergillosis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
galactomannan |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
galactosaminogalactan |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
invasive aspergillosis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
monoclonal antibody |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
mouse model |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Takazono, Takahiro
Machida, Kiyotaka
Le, Mauff Francois
Sheppard, Donald C.
Koga, Satoru
Namie Hotaka
Hishigaki, Haretsugu
Amata, Junichi
Ito, Yuya
Nakada, Nana
Hirayama, Tatsuro
Yoshida, Masataka
Takeda, Kazuaki
Iwanaga, Naoki
Ide, Shotaro
Tashiro, Masato
Hosogaya, Naoki
Sakamoto, Noriho
Matsumoto, Keitaro
Yanagihara, Katsunori
Miyazaki, Yasushi
Izumikawa, Koichi
Mukae, Hiroshi
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Background: Early diagnosis of invasive aspergillosis (IA) is critical for the initiation of effective antifungal therapy. Currently, detection of galactomannan (GM), a secreted fungal glycan, is the most used culture-independent diagnostic test for IA. However, limitations in the sensitivity and specificity of this test have led to interest in identifying other target molecules. Galactosaminogalactan (GAG), a polysaccharide cell wall component secreted by Aspergillus hyphae, is a potential diagnostic marker for IA. Objectives: To evaluate the utility of GAG as a diagnostic target, we generated a monoclonal antibody against GAG (mAb 1D1), established a GAG enzyme-linked immunosorbent assay (ELISA), evaluated its cross-reactivity with other respiratory pathogens, and compared the performance of the GAG detection ELISA with GM antigen detection in both an in vivo mouse model and human samples from patients with pulmonary aspergillosis. Results: The GAG ELISA demonstrated strong reactivity with culture supernatants from Aspergillus fumigatus and Aspergillus flavus but limited reactivity with culture supernatants of other Aspergillus spp. and non-Aspergillus filamentous fungi. In a mouse model of IA, GAG was detected in lung tissue, serum, bronchoalveolar lavage fluid (BALF), and urine samples. Although GAG was detected by mAb 1D1 staining of Aspergillus hyphae in infected human lung tissue samples, it was not detectable in the serum, BALF, and urine of patients with pulmonary aspergillosis. Conclusions: Further studies are required to determine whether the failure to detect GAG in the serum, BALF, and urine of patients with pulmonary aspergillosis is due to absence or low GAG levels or other reasons. |
|
言語 |
en |
| 書誌情報 |
en : Mycoses
巻 68,
号 10,
p. art. no. e70125,
発行日 2025-10-08
|
| 出版者 |
|
|
出版者 |
John Wiley and Sons Inc |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0933-7407 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1111/myc.70125 |
| 権利 |
|
|
権利情報 |
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. © 2025 The Author(s). Mycoses published by Wiley-VCH GmbH. |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Mycoses, 68(10), art. no. e70125; 2025 |
|
言語 |
en |
M68_e70125.pdf (2.5 MB)
