| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-10-28 |
| タイトル |
|
|
タイトル |
Usp21 Knockout Causes Abnormal Lipid Metabolism in Mouse and Its Polymorphism Correlates with Hypercholesterolemia in Outpatients |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Usp21 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Fabp7 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Nlrc5 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Ppargc1a |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
hypercholesterolemia |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Iyer, Sailakshmi
Hattori, Naoko
Okuda, Hiroshi
Nakagawa, Takeya
Fujii, Satoshi
Maeda, Takahiro
Koseki, Haruhiko
Ito, Takashi
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Usp21, a member of the ubiquitin protease family, plays a vital role in various biological functions. However, the effects of Usp21 dysfunction remain incompletely understood. In this study, we generated Usp21 knockout (KO) mice. Blood tests showed no impairment of liver function but did reveal elevated levels of total cholesterol (T-CHOL) and free fatty acid (FFA) in Usp21 KO mice compared to wild-type (WT) mice. Next, we performed RNA-sequencing (RNA-seq) to identify genes that Usp21 regulates. The results highlighted several candidate genes based on their biological relevance, and their expression levels were validated by RT-qPCR. The Usp21 KO mice exhibited significant elevations in the expression of the genes Fabp7, Nlrc5, and Ppargc1a, which play an important role in lipid metabolism, compared to WT. These data suggest that Usp21 may play roles in lipid metabolism in association with Fabp7, Nlrc5 and Ppargc1a. To clarify the involvement of USP21 in human hypercholesterolemia, we examined single-nucleotide polymorphisms (SNPs) around USP21 in non-hypercholesterolemic and hypercholesterolemic outpatients. We found that the rs11421 SNP downstream of USP21 was significantly associated with hypercholesterolemia. These data suggest that Usp21 plays a role in mice and human lipid metabolism and that its polymorphism may be a diagnostic marker for human hypercholesterolemia. |
|
言語 |
en |
| 書誌情報 |
en : International Journal of Molecular Sciences
巻 26,
号 19,
p. art. no. 9727,
発行日 2025-10-06
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| 出版者 |
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出版者 |
MDPI |
|
言語 |
en |
| ISSN |
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|
収録物識別子タイプ |
ISSN |
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収録物識別子 |
1422-0067 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.3390/ijms26199727 |
| 権利 |
|
|
権利情報 |
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/). |
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言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
International Journal of Molecular Sciences, 26(19), art. no. 9727; 2025 |
|
言語 |
en |
IJMS26_9727.pdf (1.5 MB)
