| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-11-11 |
| タイトル |
|
|
タイトル |
DNase Activity of Prevotella intermedia Impairs Biofilm Development and Neutrophil Extracellular Trap Formation |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
biofilm |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
neutrophil extracellular traps |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
periodontal disease |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
prevotella intermedia |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Seto-Tetsuo, Fumi
Ashizawa, Hiroki
Sasaki, Yuko
Yukitake, Hideharu
Shoji, Mikio
Iwanaga, Naoki
Mukae, Hiroshi
Naito, Mariko
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Chronic periodotitis is caused by the formation of biofilms. Prevotella intermedia, a gram-negative obligate anaerobic bacterium residing in periodontal pockets is involved in the formation of biofilms and secrets a highly potent DNA-degrading activity. Biofilm contains extracellular DNA as a structural component, suggesting that DNase activity may influence P. intermedia's own biofilm development. Neutrophil extracellular traps (NETs) have mesh-like structures and composed of DNA, histone and antibacterial proteins. NETs play an important role in protecting against infection, but it is possible that DNase of P. intermedia disrupts NETs. The lack of established genetic manipulation has significantly delayed the analysis of DNase pathogenic factors. Recently, we have succeeded in establishing a genetic manipulation technique for P. intermedia. In this study, we created strains lacking two DNase candidate genes, nucA (PIOMA14_I_0621) and nucD (PIOMA14_II_0624), that were highly conserved among P. intermedia strains. We examined biochemical analysis of DNase activity, their effection on biofilm formation, and their evasion of NETs. Here, we showed both of them possessed DNase activities which appeared to account all of DNase activities of the bacterium. The mutant analysis further demonstrated that NucA and NucD destroyed biofilm and NETs formations. Neither one was perfectly responsible for DNase activity, but rather they take turns depending on the conditions. In conclusion, the nucA and nucD genes encode DNases that cooperatively function on biofilm formation and suppress NETs formation in P. intermedia. |
|
言語 |
en |
| 書誌情報 |
en : MicrobiologyOpen
巻 14,
号 6,
p. art. no. e70102,
発行日 2025-10-28
|
| 出版者 |
|
|
出版者 |
John Wiley and Sons Inc |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2045-8827 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1002/mbo3.70102 |
| 権利 |
|
|
権利情報 |
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2025 The Author(s). MicrobiologyOpen published by John Wiley & Sons Ltd. |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
MicrobiologyOpen, 14(6), art. no. e70102; 2025 |
|
言語 |
en |
MO14_e70102.pdf (1.2 MB)
