| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-11-27 |
| タイトル |
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|
タイトル |
Molecular insights into nucleocapsid assembly and transport in Marburg and Ebola viruses |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Marburg virus |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Ebola virus |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
NCLS |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
live-cell imaging |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
protein structure-function |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Takamatsu, Yuki
Dolnik, Olga
Hirabayashi, Ai
Okamoto, Kenta
Kurashige, Tomomi
Shangfan, Hu
Oda, Harumi Catarina
Noda, Takeshi
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Live-cell imaging enables visualization of the spatiotemporal dynamics of signals in cells. Intracytoplasmic movement of nucleocapsids is crucial during the life cycle of enveloped viruses; however, the molecular mechanisms governing their assembly and transport are not fully understood. Using a Marburg virus (MARV) live-cell imaging system, we identified three nucleocapsid proteins—nucleoprotein (NP), VP35, and VP24—that are necessary and sufficient to form transport-competent nucleocapsid-like structures (NCLSs). These findings are consistent with observations in Ebola virus (EBOV). Interestingly, despite incompatibility among these proteins, VP30 interacts with nucleocapsid proteins from both MARV and EBOV, supporting viral transcription and replication in heterologous systems. Furthermore, we show that the conserved PPxPxY motif at the C-terminus of NP regulates NP-VP30 interactions in both homologous and heterologous contexts and is crucial for VP30 association with NCLSs. Because this motif is conserved across filoviruses, it represents a promising target for antiviral development. Our findings advance the understanding of nucleocapsid formation and offer new avenues for therapeutic intervention against MARV and EBOV. |
|
言語 |
en |
| 書誌情報 |
en : mBio
巻 16,
号 11,
p. art. no. e0155725,
発行日 2025-09-22
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| 出版者 |
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出版者 |
American Society for Microbiology |
|
言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2150-7511 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1128/mbio.01557-25 |
| 権利 |
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|
権利情報 |
© 2025 Takamatsu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
mBio, 16(11), art. no. e0155725; 2025 |
|
言語 |
en |
MB16_e0155725.pdf (3.5 MB)
