| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-12-02 |
| タイトル |
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|
タイトル |
A noninvasive test for human prion disease using hair roots and scalp |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Prion disease |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
RT-QuIC assay |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Scalp |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Biomarkers |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Hair root |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Dong, Thi-Thu-Trang
Honda, Hiroyuki
Akagi, Akio
Iwasaki, Yasushi
Kishida, Hitaru
Tsukamoto, Tadashi
Kasuga, Kensaku
Takashima, Hirotsugu
Terada, Tatsuhiro
Ikenaka, Kensuke
Ikeuchi, Takeshi
Mori, Tsuyoshi
Mochizuki, Hideki
Ono, Kenjiro
Takiyama, Yoshihisa
Hamaguchi, Tsuyoshi
Murota, Hiroyuki
Sanjo, Nobuo
Fujimoto, Takeshi
Kitayama, Michio
Fujita, Koji
Yukitake, Motohiro
Fujioka, Shinsuke
Nishida, Noriyuki
Tsuboi, Yoshio
Kitamoto, Tetsuyuki
Takao, Masaki
Yamada, Masahito
Mizusawa, Hidehiro
Satoh, Katsuya
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Invasive tests like cerebrospinal fluid (CSF) examination are highly effective for diagnosing human prion disease (HPD). Real-time quaking-induced conversion (RT-QuIC) CSF assay demonstrates 80–90% sensitivity for HPD diagnosis. To establish a minimally invasive diagnostic approach, we evaluated RT-QuIC testing on hair root and scalp samples. We collected scalp samples from 22 HPD and 5 non-HPD patients during pathological examinations and analyzed them using RT-QuIC assay and neuropathological methods. In our prospective study, hair root and CSF samples from 300 patients were tested using RT-QuIC assay along with other biomarkers, including 14-3-3 protein, total tau protein, RT-QuIC CSF assay, and MRI findings. All 22 HPD patients demonstrated positive prion seeding activity in scalp and hair root RT-QuIC assays. Neuropathological examination in one HPD patient revealed abnormal prion protein in scalp tissue. Among 177 HPD patients diagnosed by Japan Prion Surveillance Committee and 123 non-HPD patients, RT-QuIC assay of hair roots demonstrated sensitivity and specificity of 45.8% and 100%, respectively. Sensitivities of 14-3-3 protein, total tau protein, RT-QuIC CSF assay, and MRI findings were 83.1%, 86.4%, 74.6%, and 100%, respectively, with specificities of 65.0%, 65.0%, 100%, and 56.1%, respectively. RT-QuIC assays could be developed into novel diagnostic methods for neurodegenerative diseases. |
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言語 |
en |
| 書誌情報 |
en : Scientific Reports
巻 15,
号 1,
p. art. no. 41492,
発行日 2025-11-24
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| 出版者 |
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出版者 |
Springer Nature |
|
言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2045-2322 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1038/s41598-025-25310-y |
| 権利 |
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|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2025 |
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言語 |
en |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
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内容記述タイプ |
Other |
|
内容記述 |
Scientific Reports, 15(1), art. no. 41492; 2025 |
|
言語 |
en |
SR15_41492.pdf (1.3 MB)
