| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-12-09 |
| タイトル |
|
|
タイトル |
Efficacy and Limitations of Flow Cytometry for the Rapid Diagnosis of Primary Central Nervous System Lymphoma |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
flow cytometry |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
primary central nervous system lymphoma |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
diffuse large B-cell lymphoma |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Nakamura, Hikaru
Hiu, Takeshi
Kato, Takeharu
Ueki, Nozomi
Matsuo, Ayaka
Yoshida, Michiharu
Baba, Shiro
Ujifuku, Kenta
Yoshida, Koichi
Koike, Hirofumi
Hayashi, Yukishige
Hasegawa, Hiroo
Ando, Koji
Yanagihara, Katsunori
Nakashima, Masahiro
Miyazaki, Yasushi
Matsuo, Takayuki
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Background/Objectives: Primary central nervous system lymphoma (PCNSL) has a markedly high proliferation rate, making early diagnosis and prompt therapeutic intervention essential. To accelerate diagnosis, our institution adopted flow cytometry (FCM) in conjunction with conventional histopathology, and this study therefore evaluated the diagnostic performance of FCM for PCNSL. Methods: We retrospectively analyzed 67 consecutive patients with suspected PCNSL who underwent intraoperative FCM between 2010 and 2023 based on preoperative imaging. B-cell clonality was defined as ≥20% CD19/CD20-positive cells with a κ/λ ratio of >3.0 or <0.5. Results: Using histopathology, we confirmed the presence of PCNSL in 42 patients, all diagnosed as having diffuse large B-cell lymphoma. Six cases (14.3%) were discordant (FCM-D). The sensitivity, specificity, and positive predictive value of FCM were 85.7%, 100%, and 100%, respectively. T-cell markers were significantly elevated in FCM-D cases (p < 0.01), although these were pathologically diagnosed as diffuse large B-cell lymphoma based on histology and immunohistochemistry. Conclusions: FCM yields reliable diagnostic information within hours of tissue collection and supports early therapeutic decisions in PCNSL. Discordant results may reflect reactive T-cell infiltration. This is the first study to present detailed subset analyses in PCNSL using FCM in correlation with pathology, underscoring its utility as a rapid diagnostic tool. |
|
言語 |
en |
| 書誌情報 |
en : Cancers
巻 17,
号 22,
p. art. no. 3646,
発行日 2025-11-13
|
| 出版者 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2072-6694 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3390/cancers17223646 |
| 権利 |
|
|
権利情報 |
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Cancers, 17(22), art. no. 3646; 2025 |
|
言語 |
en |
C17_3646.pdf (4.8 MB)
