| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-12-18 |
| タイトル |
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|
タイトル |
One-step formation of plasmid DNA–loaded lipid–inorganic salt nanoparticles optimized via two-step design of experiments |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Gene delivery |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Plasmid DNA |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Lipids |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Calcium carbonate |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Nanoparticles |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Design of experiments |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Kato, Riku
Fumoto, Shintaro
Muttil, Pavan
Okami, Kazuya
Yamashita, Mana
Koyanagi, Hayato
Miyamoto, Hirotaka
Kawakami, Shigeru
Nishida, Koyo
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
For successful gene delivery, researchers need to consider multiple processes, such as the endosomal escape of cargos. The preparation of highly functional systems is complicated. Here, we developed a simplified preparation method for plasmid DNA–loaded galactosylated lipid–inorganic salt nanoparticles, which adopts a one-step alcohol injection method for the formation of lipid nanoparticles. The nanoparticles were designed to have a core of plasmid DNA with inorganic salts consisting of calcium carbonate and phosphate for endosomal escape and a lipid shell for the stable formation of nanoparticles. The formulation and process parameters were optimized using a two-step design of experiments, that is, a definitive screening design followed by a central composite design. We successfully established uniform nanoparticles of 120 nm in size based on micropipette mixing. Transfection efficiency of the nanoparticles prepared via micropipette mixing was equivalent to that of a commercially available transfection reagent in human hepatocellular carcinoma HepG2 cells. Using a microfluidic device, the nanoparticle size was decreased to 70 nm, while a high transfection efficiency was maintained in HepG2 cells. Moreover, the effect of inorganic salts on the nanoparticle transfection efficiency was evaluated. Notably, the formation of a calcium carbonate core was crucial for achieving an effective endosomal escape and a high transfection efficiency. Overall, this study provides valuable information for the design and simplified preparation of lipid–inorganic salt nanoparticles for efficient gene delivery. |
|
言語 |
en |
| 書誌情報 |
en : European Journal of Pharmaceutics and Biopharmaceutics
巻 219,
p. art. no. 114955,
発行日 2025-12-09
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| 出版者 |
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出版者 |
Elsevier B.V. |
|
言語 |
en |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
09396411 |
| DOI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1016/j.ejpb.2025.114955 |
| 権利 |
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|
権利情報 |
© 2025 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/. |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
European Journal of Pharmaceutics and Biopharmaceutics, 219, art. no. 114955; 2025 |
|
言語 |
en |
EJPB219_114955.pdf (1.7 MB)
