| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-01-16 |
| タイトル |
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|
タイトル |
Molecular analysis of bronchoalveolar lavage fluid to assist pulmonary lymphoma diagnoses |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Kido, Takashi
Sakamoto, Noriho
Hara, Atsuko
Kato, Takeharu
Yoshimura, Mai
Yamauchi, Shunsuke
Iketani, Chiaki
Ozasa, Mutsumi
Yura, Hirokazu
Takemoto, Shinnosuke
Takazono, Takahiro
Ishimoto, Hiroshi
Tsuruda, Kazuto
Imaizumi, Yoshitaka
Obase, Yasushi
Ishimatsu, Yuji
Nishino, Tomoya
Miyazaki, Yasushi
Yanagihara, Katsunori
Mukae, Hiroshi
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Background: Diagnosing pulmonary lymphoma is challenging and often requires surgical procedures. Molecular analysis of bronchoalveolar lavage fluid (BALF) may facilitate pulmonary lymphoma diagnosis; however, many aspects of this technique remain unknown. We aimed to establish a molecular diagnosis strategy for pulmonary lymphomas using BALF. Methods: The following molecular analyses were performed using BALF from 62 patients (eight with adult T-cell leukaemia/lymphoma (ATLL) and 11 with B-cell lymphoma): flow cytometric analysis; T-cell and B-cell clonality based on immunoglobulin heavy chain (IGH) and T-cell receptor (TCR) rearrangements; and fluorescence in situ hybridisation (FISH) analysis of IGH, mucosa-associated lymphoid tissue lymphoma translocation gene 1 (MALT1), B-cell lymphoma 2 (BCL2), and myelocytomatosis (MYC) oncogene. Results: CD25 and tumour suppressor in lung cancer-1 marker levels were significantly elevated in ATLL cases at a median of 37.8% and 33.0%, respectively, with 100% positive TCR rearrangements. Notably, FISH analysis showed numerical chromosomal abnormalities in at least one of the genes in 87.5% of patients. In B-cell lymphoma, CD19 and CD20 marker levels were significantly elevated at 4.2% and 14.3%, respectively. The sensitivity and specificity of IGH rearrangements were 81.8% and 85.4%, respectively. FISH analysis detected translocations in at least one of the genes in 45.5% of patients. Conclusions: Numerical chromosomal abnormalities in the BALF of patients with ATLL and gene translocations in B-cell lymphoma are specific and novel findings. Molecular analysis of BALF is expected to be incorporated into a diagnostic system for pulmonary lymphoma to achieve low invasiveness and improve diagnostic accuracy. |
|
言語 |
en |
| 書誌情報 |
en : ERJ Open Research
巻 11,
号 6,
p. art. no. 01377-2024,
発行日 2025-11-10
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| 出版者 |
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出版者 |
European Respiratory Society |
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言語 |
en |
| ISSN |
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|
収録物識別子タイプ |
EISSN |
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収録物識別子 |
2312-0541 |
| DOI |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1183/23120541.01377-2024 |
| 権利 |
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|
権利情報 |
© The authors 2025. This version is distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. For commercial reproduction rights and permissions contact permissions@ersnet.org |
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言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
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内容記述タイプ |
Other |
|
内容記述 |
ERJ Open Research, 11(6), art. no. 01377-2024; 2025 |
|
言語 |
en |
ERJR11_01377-2024.pdf (564 KB)
