| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-04-07 |
| タイトル |
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|
タイトル |
Distinct quaternary reaction behavior and stable charge-transfer complex formation in Schistosoma mansoni sulfide:quinone oxidoreductase |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題URI |
Sulfide metabolism |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題URI |
sulfide: quinone oxidoreductase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
UDC |
|
主題URI |
Charge-transfer complex |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題URI |
Schistosoma mansoni |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kabongo, Augustin Tshibaka
Kiplangat, Talaam Keith
Tayama, Yuki
Rajib, Acharjee
Matsuo, Yuichi
Ha, Linh Manh
Singh, Samandram Sushilkumar
Yamashita, Tetsuo
Yazaki, Euki
Hartuti, Endah Dwi
Matsunaga, Tetsuro
Ida, Tomoaki
Nozaki, Tomoyoshi
Akaike, Takaaki
Shiba, Tomoo
Kishikawa, Jun-Ichi
Hamano, Shinjiro
Kita, Kiyoshi
Inaoka, Daniel Ken
|
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Adults of Schistosoma mansoni reside in the mesenteric veins, where they are naturally exposed to high levels of hydrogen sulfide (H2S). S. mansoni and other intestinal parasites have adapted to this sulfide-rich environment, but the evolved mechanisms to metabolize sulfide remain unelucidated. Here we reveal that the putative sulfide:quinone oxidoreductase (SQOR) encoded by S. mansoni is indeed an SQOR, catalyzing the first step of sulfide metabolism. We demonstrated that S. mansoni SQOR (SmSQOR) is expressed in eggs, cercaria and adult stages and localized in the mitochondria. The reaction catalyzed by SmSQOR was investigated using sulfane sulfur probe 4 (SSP4) and shown to require the co-presence of sulfide, quinone, and a sulfur acceptor, indicating a quaternary complex-mediated mechanism. Unlike human and bacterial SQORs, purified SmSQOR could not reduce quinones in the presence of sulfide alone unless sulfite, cyanide, or L-cysteine (but not coenzyme A or glutathione) was provided as the sulfur acceptor. In the presence of these sulfur acceptors, SmSQOR formed a long-lived charge-transfer (CT) complex, a transient electronically coupled association between electron donor and acceptor, as indicated by a broad band around 637–755 nm in the spectrum, which was associated with a partial loss of enzyme activity. Moreover, residues critical for CT complex formation and SQOR catalysis were identified. Using SSP4, we also demonstrated that SQOR was active in S. mansoni adult, egg, and cercaria stages. Taken together, these features suggest that metabolism of sulfide proceeds differently in S. mansoni than in humans. |
|
言語 |
en |
| 書誌情報 |
en : Free Radical Biology and Medicine
巻 246,
p. 646-659,
発行日 2026-02-04
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| 出版者 |
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出版者 |
Elsevier Inc |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
08915849 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1016/j.freeradbiomed.2026.01.063 |
| 権利 |
|
|
権利情報 |
© 2026 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by- nc/4.0/). |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Free radical biology & medicine, 246, pp.646-659; 2026 |
|
言語 |
en |
FRBM246_646.pdf (5 MB)
