{"created":"2023-05-15T16:44:25.377288+00:00","id":20112,"links":{},"metadata":{"_buckets":{"deposit":"0da03817-986c-4cbf-a021-9ee230d3d447"},"_deposit":{"created_by":2,"id":"20112","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"20112"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00020112","sets":["29:30:1713167819629:1390"]},"author_link":["84765","84764"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"7","bibliographicPageStart":"1","bibliographicVolumeNumber":"41","bibliographic_titles":[{"bibliographic_title":"Acta medica Nagasakiensia"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In order for messenger RNA (mRNA) to be localized in cells and tissues by in situ hybridization at the electron microscopic level, the following conditions must be accomplished. The target mRNA must be retained in the specimen with well-preserved morphology. The labeled-nucleic acid probes must be accessible to the mRNA and make hybrids with the mRNA efficiently. The labels should be detectable by either transmission or scanning electron microscopes. To achieve these, appropriate techniques for the electron microscopic in situ hybridization (EMISH) for the target mRNA were developed and have been improved. This review will describe representative procedures of EMISH of mRNA and will make some comments on the procedures.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Acta medica Nagasakiensia. 1996, 41(3-4), p.1-7","subitem_description_type":"Other"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00508430","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00016055","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"P00001-P00007"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Izumi, Shin-Ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakane, Paulk."}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"acta41_03_01_t.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"acta41_03_01_t.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/20112/files/acta41_03_01_t.pdf"},"version_id":"31f216a2-24b5-4b77-a7ba-afc502a90247"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"in situ hybridization","subitem_subject_scheme":"Other"},{"subitem_subject":"electron microscopy","subitem_subject_scheme":"Other"},{"subitem_subject":"backscattered electron imaging","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"In Situ Hybridization Techniques for Electron Microscopy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In Situ Hybridization Techniques for Electron Microscopy"}]},"item_type_id":"3","owner":"2","path":["1390"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-03-13"},"publish_date":"2008-03-13","publish_status":"0","recid":"20112","relation_version_is_last":true,"title":["In Situ Hybridization Techniques for Electron Microscopy"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2024-04-17T04:05:50.219108+00:00"}