{"created":"2023-05-15T16:29:27.105767+00:00","id":202,"links":{},"metadata":{"_buckets":{"deposit":"520d3411-0097-4eab-969b-17a640845097"},"_deposit":{"created_by":2,"id":"202","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"202"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000202","sets":["35:36"]},"author_link":["926","927","924","925","929","928"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-06-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageStart":"e00109-19","bibliographicVolumeNumber":"4","bibliographic_titles":[{"bibliographic_title":"mSphere"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Babesia bovis, the most virulent causative agent of bovine babesiosis, is prevalent in tropical and subtropical regions of the world. Although the whole-genome sequence was released more than a decade ago, functional analysis of the genomics of this parasite is hampered by the limited breadth of genetic engineering tools. In this study, we implemented the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system for B. bovis and demonstrated its potential for genome editing. Cas9 and human dihydrofolate reductase (hDHFR) were simultaneously expressed by the B. bovis elongation factor-1α bidirectional promoter, and a single guide RNA was expressed via the B. bovis U6 spliceosomal RNA promoter. Using a single plasmid construct, we were able to add an epitope tag to spherical body protein 3 (SBP3), introduce a point mutation into thioredoxin peroxidase 1 (tpx-1) to impair the function of the product, and replace the tpx-1 open reading frame with the other protein. Epitope tagging of SBP3 was efficient using this system, with a negligible number of remaining wild-type parasites and a pure transgenic population produced by allelic replacement of tpx-1. This advancement in genetic engineering tools for B. bovis will aid functional analysis of the genome and underpin characterization of candidate drug and vaccine targets.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"mSphere, 4(3), e00109-19; 2019","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1128/mSphere.00109-19","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2019 Hakimi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"23795042","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hakimi, Hassan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishizaki, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kegawa, Yuto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaneko, Osamu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawazu, Shin-ichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Asada, Masahito"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"mSphere4_e00109-19.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"mSphere4_e00109-19.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/202/files/mSphere4_e00109-19.pdf"},"version_id":"54ef3cfb-51cd-41db-b587-264258a00566"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Babesia bovis","subitem_subject_scheme":"Other"},{"subitem_subject":"CRISPR/Cas9","subitem_subject_scheme":"Other"},{"subitem_subject":"genome editing","subitem_subject_scheme":"Other"},{"subitem_subject":"thioredoxin peroxidase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Genome Editing of Babesia bovis Using the CRISPR/Cas9 System","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Genome Editing of Babesia bovis Using the CRISPR/Cas9 System"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-08-02"},"publish_date":"2019-08-02","publish_status":"0","recid":"202","relation_version_is_last":true,"title":["Genome Editing of Babesia bovis Using the CRISPR/Cas9 System"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:20:08.000646+00:00"}