@article{oai:nagasaki-u.repo.nii.ac.jp:00020211,
 author = {Kondo, Shinji and Tomita, Hiroaki and Kishino, Tatsuya and Yoshiura, Kohichiro and Yamada, Koki and Soeda, Eiichi and Matsumoto, Naomichi and Ohta, Tohru and Fujii, Tohru and Niikawa, Norio},
 issue = {1-2},
 journal = {Acta medica Nagasakiensia},
 month = {Jun},
 note = {Prader-Willi syndrome (PWS) is a multiple anomalies/mental retardation syndrome. The putative PWS gene(s) remains unknown, and its occurrence is based on genomic imprinting at chromosome 15q11-q13. We have constructed a 1.5- Mb, fine, physical map of PWS critical region (PWCR) between two markers, D15S9 and D15S174 at 15q11-q13. The map is composed of 32 PAC and 3 BAC clones without any gaps. By the PAC/BAC-end sequencing procedure, a total of 26 sequence tag site (STS) markers were newly generated, and 5 expressed sequence tags (ESTs) were mapped in the region. The contig map was verified by both STS and fluorescence in situ hybridization analyses. Our map has higher resolution, compared with a previous YAC-based map of PWCR. It is useful for further genome analysis, especially on genomic imprinting of this region., Acta medica Nagasakiensia. 2000, 45(1-2), p.43-46},
 pages = {43--46},
 title = {A 1.5-Mb PAC/BAC Contig Spanning the Prader-Willi Syndrome Critical Region (PWCR)},
 volume = {45},
 year = {2000}
}