{"created":"2023-05-15T16:30:46.656122+00:00","id":2032,"links":{},"metadata":{"_buckets":{"deposit":"272ca8f3-8eb3-4cd6-b487-4bb4963c277f"},"_deposit":{"created_by":2,"id":"2032","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"2032"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00002032","sets":["10:11"]},"author_link":["9340","9341","9339","9343","9338","9342"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-06-21","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"197643","bibliographicVolumeNumber":"269","bibliographic_titles":[{"bibliographic_title":"Virus Research"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The initial phase of the human T cell leukemia virus-1 (HTLV-1) infection of salivary gland epithelial cells (SGECs) \nwas examined. SGECs of patients with Sjogren's syndrome (SS) and non-SS subjects were co-cultured with the HTLV-1-infected cell line HCT-5 or MOLT-4, then immunofluorescence (IF), scanning and transmission electron microscopy (SEM/TEM) were employed. The extracellular matrix \nand linker proteins galectin-3, agrin, and tetherin were expressed on the surfaces of both HCT-5 and MOLT-4 cells. HTLV-1 Gag-positive spots were observed on adjacent SGECs after 1 h of co-culture with HCT-5. Both in subjects with and those without SS, agrin and tetherin were co-expressed with HTLV-1 Gag on SGECs after co-culture with HCT-5,although no polarization of HTLV-1 Gag and relevant molecules was observed. SEM showed HTLV-1 virions that were found\n on HCT-5 were observed in the interfaces between HCT-5 cells and SGECs. TEM imaging showed that HTLV-1 virions were transmitted to SGECs at the interface with thin film-like structure, while HTLV-1 virions were released from the surface of HCT-5 cells. No endogenous retroviruses were observed.\n These results showed that the initial phase of HTLV-1 infection toward SGECs of SS was mediated not by viral synapses, but by biofilm-like components.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Virus Research, 269, art.no.197643; 2019","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.virusres.2019.197643","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2019 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01681702","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakamura, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimizu, Toshimasa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takatani, Ayuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suematsu, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Tatsufumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Atsushi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"VirusRes269_197643.pdf","filesize":[{"value":"2.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"VirusRes269_197643.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/2032/files/VirusRes269_197643.pdf"},"version_id":"b5800721-25e2-41dd-9f9f-0ee956e6e266"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Biofilm","subitem_subject_scheme":"Other"},{"subitem_subject":"HTLV-1","subitem_subject_scheme":"Other"},{"subitem_subject":"Salivary gland epithelial cell","subitem_subject_scheme":"Other"},{"subitem_subject":"T cell","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Initial human T-cell leukemia virus type 1 infection of the salivary gland epithelial cells requires a biofilm-like structure","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Initial human T-cell leukemia virus type 1 infection of the salivary gland epithelial cells requires a biofilm-like structure"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-06-21"},"publish_date":"2020-06-21","publish_status":"0","recid":"2032","relation_version_is_last":true,"title":["Initial human T-cell leukemia virus type 1 infection of the salivary gland epithelial cells requires a biofilm-like structure"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:53:58.596872+00:00"}