@article{oai:nagasaki-u.repo.nii.ac.jp:00020947,
 author = {Nishi, Junya and Fumoto, Shintaro and Ishii, Hiroki and Kodama, Yukinobu and Nakashima, Mikiro and Sasaki, Hitoshi and Nakamura, Junzo and Nishida, Koyo},
 issue = {2},
 journal = {European Journal of Pharmaceutics and Biopharmaceutics},
 month = {Jun},
 note = {Stomach-selective gene transfer is a promising approach as a therapeutic strategy for refractory gastric diseases. In this study, we improved the stomach selectivity of gene expression following microinstillation of naked plasmid DNA (pDNA) onto the gastric serosal surface in mice. pDNA encoding firefly luciferase was used as a reporter gene. It was confirmed that the gene expression level in the stomach 6 h after gastric serosal surface microinstillation of pDNA was significantly
higher than after intragastric, intraperitoneal and intravenous administration. Regarding selectivity of
gene expression, the gene expression level in the stomach after gastric serosal surface
microinstillation of 1 μg/1 μL (dose/volume) pDNA was 5.7 times higher than that in the spleen. In our previous study (30 μg/30 μL), the expression level in the stomach was 2.7 times higher than that in the spleen; therefore, the selectivity was 2.1 times higher in this study. When we investigated gene expression at various pDNA solution concentrations, the ratio of the gene expression level in the stomach to that in the spleen was the highest as 1 μg/1 μL of pDNA, which was considered the optimal concentration. Information in this study is useful for further development of target organ-selective gene delivery systems., European Journal of Pharmaceutics and Biopharmaceutics, 69(2), pp.633-639; 2008},
 pages = {633--639},
 title = {Improved stomach selectivity of gene expression following microinstillation of plasmid DNA onto the gastric serosal surface in mice},
 volume = {69},
 year = {2008}
}