{"created":"2023-05-15T16:45:03.615731+00:00","id":20947,"links":{},"metadata":{"_buckets":{"deposit":"b8f549c7-77cd-4ce8-8881-c8122cf4d021"},"_deposit":{"created_by":2,"id":"20947","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"20947"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00020947","sets":["73:74"]},"author_link":["88257","88258","88264","88263","88259","88262","88260","88261"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"639","bibliographicPageStart":"633","bibliographicVolumeNumber":"69","bibliographic_titles":[{"bibliographic_title":"European Journal of Pharmaceutics and Biopharmaceutics"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Stomach-selective gene transfer is a promising approach as a therapeutic strategy for refractory gastric diseases. In this study, we improved the stomach selectivity of gene expression following microinstillation of naked plasmid DNA (pDNA) onto the gastric serosal surface in mice. pDNA encoding firefly luciferase was used as a reporter gene. It was confirmed that the gene expression level in the stomach 6 h after gastric serosal surface microinstillation of pDNA was significantly\nhigher than after intragastric, intraperitoneal and intravenous administration. Regarding selectivity of\ngene expression, the gene expression level in the stomach after gastric serosal surface\nmicroinstillation of 1 μg/1 μL (dose/volume) pDNA was 5.7 times higher than that in the spleen. In our previous study (30 μg/30 μL), the expression level in the stomach was 2.7 times higher than that in the spleen; therefore, the selectivity was 2.1 times higher in this study. When we investigated gene expression at various pDNA solution concentrations, the ratio of the gene expression level in the stomach to that in the spleen was the highest as 1 μg/1 μL of pDNA, which was considered the optimal concentration. Information in this study is useful for further development of target organ-selective gene delivery systems.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"European Journal of Pharmaceutics and Biopharmaceutics, 69(2), pp.633-639; 2008","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ejpb.2007.12.018","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright c 2007 Elsevier B.V. All rights reserved."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10836618","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09396411","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nishi, Junya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fumoto, Shintaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishii, Hiroki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kodama, Yukinobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakashima, Mikiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasaki, Hitoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Junzo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishida, Koyo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"EJPB69_633.pdf","filesize":[{"value":"286.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"EJPB69_633.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/20947/files/EJPB69_633.pdf"},"version_id":"10293efa-69ba-4617-9800-25b0f0700724"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Gene delivery","subitem_subject_scheme":"Other"},{"subitem_subject":"Targeting","subitem_subject_scheme":"Other"},{"subitem_subject":"Stomach","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene therapy","subitem_subject_scheme":"Other"},{"subitem_subject":"Plasmid DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"Mouse","subitem_subject_scheme":"Other"},{"subitem_subject":"Luciferase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Improved stomach selectivity of gene expression following microinstillation of plasmid DNA onto the gastric serosal surface in mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Improved stomach selectivity of gene expression following microinstillation of plasmid DNA onto the gastric serosal surface in mice"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-06-02"},"publish_date":"2008-06-02","publish_status":"0","recid":"20947","relation_version_is_last":true,"title":["Improved stomach selectivity of gene expression following microinstillation of plasmid DNA onto the gastric serosal surface in mice"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:14:23.969647+00:00"}