@article{oai:nagasaki-u.repo.nii.ac.jp:00002097,
 author = {Nishiguchi, Tomoki and Cho, Kichul and Isaka, Shogo and Ueno, Mikinori and Jin, Jun-O and Yamaguchi, Kenichi and Kim, Daekyung and Oda, Tatsuya},
 issue = {Part A},
 journal = {International Journal of Biological Macromolecules},
 month = {Dec},
 note = {Porphyran, a sulfated polysaccharide, isolated from discolored nori (Porphyra yezoensis) (dc-porphyran) and one fraction (F1) purified from dc-porphyran by DEAE-chromatography showed the protective effects on LPS-induced endotoxin shock in mice. Intraperitoneal (i.p.) treatment with dc-porphyran or F1 (100 mg/kg) 60 min prior to i.p. injection of LPS (30 mg/kg) completely protected mice from LPS lethality. At 10 mg/kg concentration, F1 demonstrated more protection than dc-porphyran. Intravenous (i.v.) challenge of LPS, even at 20 mg/kg, was more lethal than i.p. administration; i.v. injection of F1 (100 mg/kg) with LPS significantly improved the survival rate. However, i.v. dc-porphyran (100 mg/kg) produced an even lower survival rate than that of LPS alone. We examined pro-inflammatory mediators such as NO and TNF-α in serum. F1 significantly reduced the levels of these markers. Additionally, F1 significantly decreased the malondialdehyde level in the liver, a marker of oxidative stress, while dc-porphyran had almost no effect. Furthermore, F1 significantly decreased the production of TNF-α and NO in peritoneal exudate cells harvested from LPS-challenged mice, while dc-porphyran treatment showed a lesser decrease. Our results suggest that porphyran isolated from discolored nori, especially F1, is capable of suppressing LPS-induced endotoxin shock in vivo., International Journal of Biological Macromolecules, 93(Part A), pp.1273-1278; 2016},
 pages = {1273--1278},
 title = {Protective effect of porphyran isolated from discolored nori (Porphyra yezoensis) on lipopolysaccharide-induced endotoxin shock in mice},
 volume = {93},
 year = {2016}
}