{"created":"2023-05-15T16:30:49.746570+00:00","id":2103,"links":{},"metadata":{"_buckets":{"deposit":"8e165fd7-f99d-4763-955f-6d882bc43f2f"},"_deposit":{"created_by":2,"id":"2103","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"2103"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00002103","sets":["73:74"]},"author_link":["9723","9724","9726","9722","9728","9725","9727"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-10-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"1738","bibliographicPageStart":"1734","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"Biological & Pharmaceutical Bulletin"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We previously developed a negatively charged amino acid dendrimer to address the safety concerns associated with the constituent unit of these systems, which resulted in the formation of a sixth-generation glutamic acid-modified dendritic poly(L-lysine) system (KG6E). The aim of this study was to develop a nanocarrier for targeted drug delivery into cancer cells. In this study, we have synthesized a conjugate material consisting of anti-mucin 1 (MUC1) aptamer (anti-MUC1 apt) and KG6E (anti-MUC1 apt/KG6E) for targeted drug delivery to human lung adenocarcinoma A549 cells, which express high levels of the MUC1. The anti-MUC1 apt/KG6E was efficiently internalized by the A549 cells and subsequently transported to the endosomal and lysosomal compartments. In contrast, the cellular association of the sequence scrambled aptamer/KG6E conjugate (scrambled apt/KG6E) was much lower than that of the anti-MUC1 apt/KG6E in A549 cells. These results suggest that our newly developed anti-MUC1 apt/KG6E can be internalized in A549 cells via a MUC1 recognition pathway.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biological & Pharmaceutical Bulletin, 39(10), pp.1734-1738; 2016","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本薬学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1248/bpb.b16-00508","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2016 The Pharmaceutical Society of Japan."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09186158","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13475215","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Pharmaceutical Society of Japan"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Masuda, Marie"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Shigeru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wijagkanalan, Wassana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suga, Tadaharu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fuchigami, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Fumiyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashida, Mitsuru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"BPBul39_1734.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BPBul39_1734.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/2103/files/BPBul39_1734.pdf"},"version_id":"e7de076a-ed55-41b8-94a1-6c700429f548"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Aptamer","subitem_subject_scheme":"Other"},{"subitem_subject":"Dendrimer","subitem_subject_scheme":"Other"},{"subitem_subject":"Drug delivery","subitem_subject_scheme":"Other"},{"subitem_subject":"Mucin 1 (MUC1)","subitem_subject_scheme":"Other"},{"subitem_subject":"Targeting","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Anti-MUC1 Aptamer/Negatively Charged Amino Acid Dendrimer Conjugates for Targeted Delivery to Human Lung Adenocarcinoma A549 Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Anti-MUC1 Aptamer/Negatively Charged Amino Acid Dendrimer Conjugates for Targeted Delivery to Human Lung Adenocarcinoma A549 Cells"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-05-16"},"publish_date":"2017-05-16","publish_status":"0","recid":"2103","relation_version_is_last":true,"title":["Anti-MUC1 Aptamer/Negatively Charged Amino Acid Dendrimer Conjugates for Targeted Delivery to Human Lung Adenocarcinoma A549 Cells"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:52:39.794330+00:00"}