{"created":"2023-05-15T16:30:52.447213+00:00","id":2158,"links":{},"metadata":{"_buckets":{"deposit":"27a8673f-169f-4015-8f04-83badabc7d71"},"_deposit":{"created_by":2,"id":"2158","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"2158"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00002158","sets":["10:11"]},"author_link":["10016","10022","10020","10021","10018","10017","10019","10023"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-09-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"44","bibliographicPageEnd":"71273","bibliographicPageStart":"71255","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"Oncotarget"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The mechanism by which type II interferon (IFN) inhibits virus replications remains to be identified. Murine leukemia virus (MLV) replication was significantly restricted by γ-IFN, but not human immunodeficiency virus type 1 (HIV-1) replication. Because MLV enters host cells via endosomes, we speculated that certain cellular factors among γ-IFN-induced, endosome-localized proteins inhibit MLV replication. We found that γ-IFN-inducible lysosomal thiolreductase (GILT) significantly restricts HIV- 1 replication as well as MLV replication by its thiolreductase activity. GILT silencing enhanced replication-defective HIV-1 vector infection and virion production in γ-IFNtreated cells, although γ-IFN did not inhibit HIV-1 replication. This result showed that GILT is required for the anti-viral activity of γ-IFN. Interestingly, GILT protein level was increased by γ-IFN in uninfected cells and env-deleted HIV-1-infected cells, but not in full-length HIV-1-infected cells. γ-IFN-induced transcription from the γ-IFNactivation sequence was attenuated by the HIV-1 Env protein. These results suggested that the γ-IFN cannot restrict HIV-1 replication due to the inhibition of γ-IFN signaling by HIV-1 Env. Finally, we found that 4,4'-dithiodipyridine (4-PDS), which inhibits S-S bond formation at acidic pH, significantly suppresses HIV-1 vector infection and virion production, like GILT. In conclusion, this study showed that GILT functions as a host restriction factor against the retroviruses, and a GILT mimic, 4-PDS, is the leading compound for the development of novel concept of anti-viral agents.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Oncotarget, 7(44), pp.71255-71273; 2016","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Impact Journals LLC"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.18632/oncotarget.12104","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2016 Impact Journals, LLC"},{"subitem_rights":"This article is licensed under a Creative Commons Attribution 3.0 License."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19492553","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kubo, Yoshinao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Izumida, Mai"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yashima, Yuka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshii-Kamiyama, Haruka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Yuetsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yasui, Kiyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hayashi, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuyama, Toshifumi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"Onco7_71255.pdf","filesize":[{"value":"4.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Onco7_71255.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/2158/files/Onco7_71255.pdf"},"version_id":"e5ccedb4-cee3-4907-a702-cb8c831b7ec2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Antiviral","subitem_subject_scheme":"Other"},{"subitem_subject":"Endosome","subitem_subject_scheme":"Other"},{"subitem_subject":"Gamma-interferon","subitem_subject_scheme":"Other"},{"subitem_subject":"Retroviruses","subitem_subject_scheme":"Other"},{"subitem_subject":"Thiolreductase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Gamma-interferon-inducible, lysosome/endosome-localized thiolreductase, GILT, has anti-retroviral activity and its expression is counteracted by HIV-1","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Gamma-interferon-inducible, lysosome/endosome-localized thiolreductase, GILT, has anti-retroviral activity and its expression is counteracted by HIV-1"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-05-25"},"publish_date":"2017-05-25","publish_status":"0","recid":"2158","relation_version_is_last":true,"title":["Gamma-interferon-inducible, lysosome/endosome-localized thiolreductase, GILT, has anti-retroviral activity and its expression is counteracted by HIV-1"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:51:48.721321+00:00"}