@article{oai:nagasaki-u.repo.nii.ac.jp:00021591, author = {Matsuda, Genji and Maita, Tetsuo and Takei, Hiroshi and Maekawa, Tomoyuki and Shikaya, Takao and Maeda, Kenji and Fujiwara, Masaroku}, issue = {1-2}, journal = {Acta medica Nagasakiensia}, month = {Dec}, note = {The technique of adsorption chromatography of dinitrophenylamino acids (DNP-amino acids) has been studied in this laboratory in order to separate N-terminal amino acids from the globins of various hemoglobins. The following method which gave good results is a modified method of GREEN et al. A mixture of silica gel and celite (2 to 1) is packed into a 15-cm. column with diameter of 0.9 cm. As developers mixtures of organic solvents such as acetic acid, acetone, ligroin, formic acid, ethyl acetate or benzene are used. In the above column and with the above developers, adsorption chromatography is carried out to separate various DNP-amino acids. This seems to be an effective method for qualitative and quantitative analysis of DNA-amino acids., Acta medica Nagasakiensia. 1963, 8(1-2), p.28-34}, pages = {28--34}, title = {Adsorption Chromatography of Dinitrophenylamino Acids on Silica Gel-Celite}, volume = {8}, year = {1963} }