@article{oai:nagasaki-u.repo.nii.ac.jp:00021780,
 author = {Ishibashi, Tsunehisa},
 issue = {1-3},
 journal = {Acta medica Nagasakiensia},
 month = {Oct},
 note = {The cellular DNA and RNA contents of the lymphocytes were assessed by using a Fluorescence Activating Cell Sortor System (FACS-IV)to evaluate the lymphocyte response to the lectin stimulation or the operative insult . 1) The DNA and RNA contents in the lymphocytes stimulated by the lectins such as phytohemagglutinin (PHA), Concanavalin A (Con A) and Pokeweed mitogen (PWM) were measured in vitro by using the technique of FACS-IV in comparision with those by the uptakes of 3H-thymidine dR and Uridine R . The DNA and RNA syntheses began to start at the first 6 hours of stimulation by the lectins in accordance with an appearance of the lymphocyte in early S phase and it was apparent that mitosis occurred for a period between 36 and 48 hours . It is to be further emphasized that DNA syntheses in the lectin-stimulated lymphocytes were initiated with the passage of 6 hours and remarkably increasing as those at 12 and 24 hr . 2) Postoperative states were evaluated from the standpoint of change in the cellular RNA contents in the monocytes. An increase in the RNA content was noticeable on day 1 to day 7 following surgery and these changes were associated with postoperatively serious conditions such as either grave complication or major operative invasion . In conclusion, the assessment of the cellular RNA content in the monocytes plays an important role in identifying postoperative state from serious condition on the course of postoperative period., Acta medica Nagasakiensia. 1985, 30(1-3), p.225-237},
 pages = {225--237},
 title = {Kinetic Changes of DNA and RNA Contents in Activated Lymphocytes upon Lectin Stimulation and Operative Insult},
 volume = {30},
 year = {1985}
}