{"created":"2023-05-15T16:45:46.855731+00:00","id":21950,"links":{},"metadata":{"_buckets":{"deposit":"af678dd4-70c4-4ab8-8e65-ce8fd85cc477"},"_deposit":{"created_by":2,"id":"21950","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"21950"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00021950","sets":["29:30:1713167819629:1561"]},"author_link":["93652","93650","93647","93646","93648","93649","93651"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1990-12-14","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-4","bibliographicPageEnd":"303","bibliographicPageStart":"298","bibliographicVolumeNumber":"35","bibliographic_titles":[{"bibliographic_title":"Acta medica Nagasakiensia"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We carried out investigations on muscarinic acetylcholine receptors (m-AChR) in the rat heart, including the atrioventricular (AV) node. The related tissue sections were incubated with 3H-quinuclidinyl benzilate (3H-QNB), then autoradiography and an image analysis coupled with computer-assisted microdensitometry were done. A single type of specific and high affinity binding sites of 3H-QNB was found to be highly concentrated in the AV node, the maximum binding capacity (Bmax) being 1.4 pmol/mg protein and with a dissociation constant (Kd) of 0.5 nM. The density and affinity of the binding to the AV node were the highest, when compared with findings in the atrium (interatrial septum) and ventricle (interventricular septum). The binding was competitively displaced by AF-DX 116, a selective antagonist for the M2 AChR subtype, with a high affinity, whereas pirenzepine, an antagonist for the M1 AChR subtype was much less potent in displacing the binding. Therefore, vagal-cholinergic stimulation presumably plays a significant role in functions of the rat AV node, probably by interacting with the specific, high affinity M2 AChR subtype.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Acta medica Nagasakiensia. 1990, 35(1-4), p.298-303","subitem_description_type":"Other"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00508430","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00016055","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"P00298-P00303"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamasaki, Hidefumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Niwa, Masami"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shigematsu, Kazuto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Seto, Shinji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oku, Yasuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ozaki, Masayori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashiba, Kunitake"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"acta35_01_55_t.pdf","filesize":[{"value":"554.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"acta35_01_55_t.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/21950/files/acta35_01_55_t.pdf"},"version_id":"c0f0294b-7a83-4b52-8094-4b874a645d80"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Receptor Autoradiographic Analysis of Muscarinic Receptors in the Rat Atrioventricular Node","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Receptor Autoradiographic Analysis of Muscarinic Receptors in the Rat Atrioventricular Node"}]},"item_type_id":"3","owner":"2","path":["1561"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-03-13"},"publish_date":"2008-03-13","publish_status":"0","recid":"21950","relation_version_is_last":true,"title":["Receptor Autoradiographic Analysis of Muscarinic Receptors in the Rat Atrioventricular Node"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2024-04-17T04:10:10.342521+00:00"}