{"created":"2023-05-15T16:46:19.866060+00:00","id":22584,"links":{},"metadata":{"_buckets":{"deposit":"892a183d-79e7-4b8a-95c9-f2e4bafe7594"},"_deposit":{"created_by":2,"id":"22584","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"22584"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00022584","sets":["29:30:1713167819629:1609"]},"author_link":["96070","96071","96073","96072"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"supl.1","bibliographicPageEnd":"54","bibliographicPageStart":"51","bibliographicVolumeNumber":"50","bibliographic_titles":[{"bibliographic_title":"Acta medica Nagasakiensia"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"It is well established that tumor suppressor p53 protein is stabilized and activated as a sequence-specific transcriptional factor in response to genotoxic stresses, including ionizing radiation (IR). Previous studies demonstrated that p53 is phosphorylated at N-terminal serines and a threonine after IR. Here, we examined the role of the phosphorylation, especially at Ser15, Thr18, and Ser20, in IR-induced p53 stabilization and activation, using alanine-substituted p53 at phosphorylation site (s). In the present study, we examined p53 stabilization and activation in two different cell lines : stabilization in HT1080-derived clone in which endogenous wild-type p53 was confirmed to be normally stabilized after IR; activation in p53-null H1299-derived clone in which ectopically-expressed p53 levels did not change before and after IR, because the increase in p53 levels affects transactivation by p53. In Western blot analysis and immunofluorescence staining, alanine-substituted p53 at Ser15, Thr18, and/or Ser20 was stabilized in the nucleus comparably with wild-type p53 2 h after 4 Gy of X-rays. However, Ser15-alanine-substituted p53 did not induce the expression of p21 protein, one of the p53-targeted gene products, after IR. These results indicate that phosphorylation at Ser15, Thr18, and Ser20 is not required for IR-induced p53 stabilization, however, Ser15-phosphorylation plays a role in IR-induced p21 induction by p53.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Acta medica Nagasakiensia. 2005, 50(supl.1), p.51-54","subitem_description_type":"Other"}]},"item_3_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://joi.jlc.jst.go.jp/JST.JSTAGE/amn/50.S51"}]}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00508430","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00016055","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"P00051-P00054"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamauchi, Motohiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Keiji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kodama, Seiji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Masami"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-24"}],"displaytype":"detail","filename":"acta50_s1_51.pdf","filesize":[{"value":"463.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"acta50_s1_51.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/22584/files/acta50_s1_51.pdf"},"version_id":"4dc5a492-5b56-4a55-b0b5-d2c3a7c376fd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"p53","subitem_subject_scheme":"Other"},{"subitem_subject":"Phosphorylation","subitem_subject_scheme":"Other"},{"subitem_subject":"Stabilization","subitem_subject_scheme":"Other"},{"subitem_subject":"Activation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Role of Phosphorylation in IR-induced p53 Stabilization and Activation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Role of Phosphorylation in IR-induced p53 Stabilization and Activation"}]},"item_type_id":"3","owner":"2","path":["1609"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-11-02"},"publish_date":"2007-11-02","publish_status":"0","recid":"22584","relation_version_is_last":true,"title":["The Role of Phosphorylation in IR-induced p53 Stabilization and Activation"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2024-04-17T03:58:50.768880+00:00"}