{"created":"2023-05-15T16:46:21.595298+00:00","id":22624,"links":{},"metadata":{"_buckets":{"deposit":"f1d493ea-a212-4dc0-ace5-d0418e20d866"},"_deposit":{"created_by":2,"id":"22624","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"22624"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00022624","sets":["29:30:1713167819629:1614"]},"author_link":["96276","96273","96274","96275","96272","96277"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"17","bibliographicPageStart":"9","bibliographicVolumeNumber":"51","bibliographic_titles":[{"bibliographic_title":"Acta medica Nagasakiensia"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Angiotensin II (Ang-II) and connective tissue growth factor (CTGF) are involved in various renal disorders that lead to end-stage renal disease. Here, we determined the role of Ang-II and CTGF in the progression of tubulointerstitial injury in the rat unilateral ureteral obstruction (UUO) model. Sprague-Dawley rats (n=16) were used; 10 rats underwent UUO, and 6 control rats underwent sham operation; and rats of both groups were sacrificed on days 7 or 14. Histomorphometric analysis was performed to quantitate tubulointerstitial injuries in the experimental group. Kidney sections were stained immunohistochemically for Ang-II, CTGF, transforming growth factor-β1 (TGF-β1), type III collagen and α-smooth muscle actin (α-SMA). Renal CTGF expression was studied using in situ hybridization and reverse transcriptase-polymerase chain reaction. Double staining for Ang-II with α-SMA and CTGF with α-SMA was performed to identify cells with enhanced expression of Ang-II and CTGF. Similar dual staining of Ang-II with type III collagen and CTGF with type III collagen was performed. The correlation between Ang-II and CTGF expression and tubulointerstitial injury was examined. In obstructed kidneys, there was a significant (p<0.001) increase in expression of Ang-II, CTGF, TGF-β1, type III collagen and α-SMA, compared with control kidneys. Tubular epithelial cells and interstitial cells were the main Ang-II- and CTGF-producing cells in the obstructed kidneys. A significantly (p<0.001) positive correlation was detected in obstructed kidneys between renal expression of CTGF and expression of TGF-β1 (r=0.91), type III collagen (r=0.87) or α-SMA (r=0.90). Similarly a significantly (p<0.001) positive correlation was found in obstructed kidneys between Ang-II expression and expression of TGF-β1 (r=0.88), type III collagen (r=0.79) and α-SMA (r=0.91). Finally, there were significantly positive correlations between CTGF /Ang-II expression and tubulointerstitial fibrosis in the obstructed kidneys (r=0.88, p<0.001). The results of our in vivo studies suggest that both Ang-II and CTGF, produced by intrarenal cells, coordinately regulate progression of renal tubulointerstitial injury, by facilitating increased accumulation of interstitial collagens in obstructed kidneys.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Acta medica Nagasakiensia. 2006, 51(1), p.9-17","subitem_description_type":"Other"}]},"item_3_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://joi.jlc.jst.go.jp/JST.JSTAGE/amn/51.9"}]}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00508430","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00016055","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"P00009-P00017"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Le, VietThang"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shigematsu, Kazuto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zha, Yan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nazneen, Arifa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"S.Razzaque, Mohammed"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taguchi, Takashi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-24"}],"displaytype":"detail","filename":"acta51_01_09.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"acta51_01_09.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/22624/files/acta51_01_09.pdf"},"version_id":"c66b9fc8-62cd-4746-b94b-ef7784d1d78c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Obstructivenephropathy","subitem_subject_scheme":"Other"},{"subitem_subject":"Unilateralureteralobstruction","subitem_subject_scheme":"Other"},{"subitem_subject":"CTGF","subitem_subject_scheme":"Other"},{"subitem_subject":"AngiotensinII","subitem_subject_scheme":"Other"},{"subitem_subject":"Tubulointerstitialfibrosis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Role of Connective Tissue Growth Factor and Angiotensin II in Tubulointerstitial Fibrosis in Experimental Obstructive Nephropathy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Role of Connective Tissue Growth Factor and Angiotensin II in Tubulointerstitial Fibrosis in Experimental Obstructive Nephropathy"}]},"item_type_id":"3","owner":"2","path":["1614"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-11-02"},"publish_date":"2007-11-02","publish_status":"0","recid":"22624","relation_version_is_last":true,"title":["Role of Connective Tissue Growth Factor and Angiotensin II in Tubulointerstitial Fibrosis in Experimental Obstructive Nephropathy"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2024-04-16T05:36:11.840303+00:00"}