{"created":"2023-05-15T16:31:03.562135+00:00","id":2363,"links":{},"metadata":{"_buckets":{"deposit":"37c970ca-c8fa-4dfd-8bab-bf480acd3d97"},"_deposit":{"created_by":6,"id":"2363","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"2363"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00002363","sets":["10:22:24"]},"author_link":["115758","115757"],"item_5_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"TGF-s1の過剰発現と低酸素状態は不動に伴う皮膚の線維化の発生と進行に関与する―膝関節屈曲拘縮モデルラットにおける検討―"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03-21","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"596","bibliographicPageStart":"586","bibliographicVolumeNumber":"58","bibliographic_titles":[{"bibliographic_title":"Connective Tissue Research"}]}]},"item_5_date_granted_64":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2017-03-21"}]},"item_5_degree_grantor_62":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Nagasaki University (長崎大学)"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17301","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_61":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: The purpose of this study was to investigate the pathology and mechanism of immobilization-induced skin fibrosis in a rat joint contracture model. Methods: Rats were randomly divided into control and immobilization groups. In the immobilization groups, knee joints of the rats were immobilized for 1, 2, and 4 weeks. After each immobilization, skin was dissected. To assess fibrosis in the skin, the thickness and area of adipocytes and connective tissue fibers were measured. Myofibroblasts were analyzed by immunohistochemistry by using anti-α-SMA as a marker. Gene expression levels of type I and III collagen, TGF-s1, and HIF-1α were measured by using RT-PCR. Results: One week after immobilization, there was a marked increase in the area of connective tissue fibers in the immobilization group. Type I and type III collagen were significantly increased with prolonged immobilization. Higher numbers of α-SMA-positive cells were noted in the immobilized group at 2 and 4 weeks after immobilization. The expression level of TGF-β1 mRNA in the immobilization group increased after one week of immobilization. In contrast, the expression level of HIF1-α mRNA increased after 2 weeks of immobilization, and a greater increase was seen at 4 weeks after immobilization. Conclusions: These results suggest that immobilization induces skin fibrosis with accumulation of types I and III collagen. These fibrotic changes may be evoked by upregulation of TGF-β1 after one week of immobilization. Additionally, upregulation of HIF-1α may relate to skin fibrosis by accelerating the differentiation of fibroblasts to myofibroblasts starting at 2 weeks after immobilization.","subitem_description_type":"Abstract"}]},"item_5_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"長崎大学学位論文 学位記番号:博(医歯薬)甲第953号 学位授与年月日:平成29年3月21日","subitem_description_type":"Other"},{"subitem_description":"Author: Kyo Goto, Junya Sakamoto, Jiro Nakano, Hideki Kataoka, Yuichiro Honda, Ryo Sasabe, Tomoki Origuchi & Minoru Okita","subitem_description_type":"Other"},{"subitem_description":"Citation: Connective Tissue Research, 58(6), pp.586-596; 2017","subitem_description_type":"Other"}]},"item_5_description_69":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Nagasaki University (長崎大学), 博士(医学) (2017-03-21)","subitem_description_type":"Other"}]},"item_5_dissertation_number_65":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医歯薬第953号"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"115758","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Goto, Kyo"}]}]},"item_5_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Taylor and Francis Ltd"}]},"item_5_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1080/03008207.2017.1284823","subitem_relation_type_select":"DOI"}}]},"item_5_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10069/37411","subitem_relation_type_select":"HDL"}}]},"item_5_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2017 Taylor & Francis"},{"subitem_rights":"This is an Accepted Manuscript of an article published by Taylor & Francis in Connective Tissue Research on 26 Feb 2017, available online: http://www.tandfonline.com/10.1080/03008207.2017.1284823."}]},"item_5_text_67":{"attribute_name":"学位の種類","attribute_value_mlt":[{"subitem_text_value":"課程博士"}]},"item_5_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"後藤, 響"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-02-01"}],"displaytype":"detail","filename":"ISYK953_Goto.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ISYK953_Goto.pdf","objectType":"fulltext","url":"https://nagasaki-u.repo.nii.ac.jp/record/2363/files/ISYK953_Goto.pdf"},"version_id":"a6b5813f-1ec6-431a-b117-8d3ec622fbbe"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hypoxia","subitem_subject_scheme":"Other"},{"subitem_subject":"immobilization","subitem_subject_scheme":"Other"},{"subitem_subject":"collagen","subitem_subject_scheme":"Other"},{"subitem_subject":"myofibroblast","subitem_subject_scheme":"Other"},{"subitem_subject":"skin fibrosis","subitem_subject_scheme":"Other"},{"subitem_subject":"transforming growth factor-β1","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Development and progression of immobilization-induced skin fibrosis through overexpression of transforming growth factor-s1 and hypoxic conditions in a rat knee joint contracture model.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development and progression of immobilization-induced skin fibrosis through overexpression of transforming growth factor-s1 and hypoxic conditions in a rat knee joint contracture model.","subitem_title_language":"en"}]},"item_type_id":"5","owner":"6","path":["24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-06-22"},"publish_date":"2017-06-22","publish_status":"0","recid":"2363","relation_version_is_last":true,"title":["Development and progression of immobilization-induced skin fibrosis through overexpression of transforming growth factor-s1 and hypoxic conditions in a rat knee joint contracture model."],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-08-23T04:56:16.064946+00:00"}