{"created":"2023-05-15T16:31:05.908118+00:00","id":2417,"links":{},"metadata":{"_buckets":{"deposit":"f1814e7b-9ac1-4fdf-b5f2-0cf000e99425"},"_deposit":{"created_by":2,"id":"2417","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"2417"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00002417","sets":["50:51"]},"author_link":["11268","11267","11271","11265","11270","11266","11269"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-11","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"406","bibliographicPageStart":"400","bibliographicVolumeNumber":"104","bibliographic_titles":[{"bibliographic_title":"International Journal of Biological Macromolecules"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Our previous studies demonstrated that the microalga Parachlorella kessleri (KNK-A001) has immunostimulatory activities, which were observed as an increase in natural killer (NK) cell activity in mice after intraperitoneal injection or as a protective effect on a virus-infected model shrimp after oral administration. In this study, we attempted to gain insight into the constituent substances of KNK-A001 that are responsible for the immunostimulatory activity. First, we obtained five polysaccharide fractions from KNK-A001 by DEAE anion exchange chromatography. Among the fractions, F5 showed the most potent induction of nitric oxide (NO) secretion in RAW264.7 cells, and both mRNA and protein expression levels of inducible NO synthase (iNOS) were increased in F5-treated RAW264.7 cells. A significant increase in the nuclear translocation of the p65 subunit of nuclear factor-kappa B (NF-κB) was observed in F5-treated RAW264.7 cells. F5 also induced the secretion of tumor necrosis factor (TNF)-α in RAW264.7 cells. Analysis using mitogen-activated protein (MAP) kinase inhibitors suggested that c-Jun N-terminal kinase (JNK) and p38 MAP kinase were mainly involved in F5-induced NO and TNF-α productions. The compositional analysis of F5 identified the main constituents as galactose, glucose, galacturonic acid, and mannose. Gel-filtration analysis suggested that molecular mass of F5 was approximately 400 kDa.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Biological Macromolecules, 104, pp.400-406; 2017","subitem_description_type":"Other"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ijbiomac.2017.06.014","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2017 Elsevier B.V. All rights reserved."},{"subitem_rights":"This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01418130","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ueno, Mikinori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Cho, Kichul"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirata, Narumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Kenji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Kenichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kim, Daekyung"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oda, Tatsuya"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"IJBM104_400.pdf","filesize":[{"value":"348.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJBM104_400.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/2417/files/IJBM104_400.pdf"},"version_id":"8f122a22-7f08-4e09-9a86-a3a29d296e05"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Macrophages","subitem_subject_scheme":"Other"},{"subitem_subject":"Nitric oxide","subitem_subject_scheme":"Other"},{"subitem_subject":"Parachlorella kessleri","subitem_subject_scheme":"Other"},{"subitem_subject":"Polysaccharides","subitem_subject_scheme":"Other"},{"subitem_subject":"NF-κB","subitem_subject_scheme":"Other"},{"subitem_subject":"TNF-α","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Macrophage-stimulating activities of newly isolated complex polysaccharides from  Parachlorella kessleri  strain KNK-A001","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Macrophage-stimulating activities of newly isolated complex polysaccharides from  Parachlorella kessleri  strain KNK-A001"}]},"item_type_id":"2","owner":"2","path":["51"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-12-01"},"publish_date":"2018-12-01","publish_status":"0","recid":"2417","relation_version_is_last":true,"title":["Macrophage-stimulating activities of newly isolated complex polysaccharides from  Parachlorella kessleri  strain KNK-A001"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:47:13.018736+00:00"}