@article{oai:nagasaki-u.repo.nii.ac.jp:00024687,
 author = {宇都宮, 明剛},
 issue = {3},
 journal = {熱帯医学 Tropical medicine},
 month = {Dec},
 note = {The experimental cholera in infantile mice by oral challenge with El Tor Inaba type V86 which was investigated by Ujiiye et al. (16), showed that the susceptibility to the infection was closely related to the day of life; in mice younger than 10 days old, a retention of fluid and multiplication of vibrios in the intestine were seen 24 hours after the challenge. However, in 15 days old mice, no retention of fluid in the intestine was found and challenged vibrios were not so multiplied as in the younger; neither retention of fluid nor multiplication of vibrios was recognized in 30 days old mice. Then, the infancy was considered one of the important host factors in the pathogenesis of experimental cholera of mice. It was supposed that the infancy would include various kinds of factors which should be related to the pathogenesis of experimental cholera. Among them, an intestinal flora, particularly coli flora was assumed to play significant role for the infection. In the present investigation, coliform organisms appeared usually about 8 days after the birth in the intestine of mice and then, the intestinal flora was going to be gradually established. It was observed that the number of coliform organisms decreased extremely and finally disappeared in the intestine of mice, when streptomycin was given continuously with drinking water. On the other hand, streptomycin-resistant strain of El Tor Inaba V86 was able to be isolated as one step mutant on the streptomycin-containing medium. Streptomycin-resistant V86 was orally challenged on mice, of which in the intestine, no or few coliform organisms were found. It was noticed that streptomycin-resistant V86 was multiplied in the intestine even in such adult mice, although no retention of fluid was seen in the intestine. It was presumed that the coli flora in the intestine would play a protective role against the multiplication of vibrios which was orally administered into the intestine, although it could not be denied that the coli flora was only an indicator which revealed a particular condition changed by streptomycin. The significance of the intestinal, particularly coli flora should be investigated in further studies., 熱帯医学 Tropical medicine 11(3). p170-182, 1969},
 pages = {170--182},
 title = {マウスのコレラ菌経口感染実験に対する腸内大腸菌叢の影響},
 volume = {11},
 year = {1969}
}