{"created":"2023-05-15T16:47:57.633170+00:00","id":24815,"links":{},"metadata":{"_buckets":{"deposit":"1e6875a5-61e6-491e-8fa2-786f4dba1908"},"_deposit":{"created_by":2,"id":"24815","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"24815"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00024815","sets":["35:1741:1775:1810"]},"author_link":["104439","104438","104441","104440","104442","104443"],"item_3_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Plaque Assay Method for Adenovirus Type 5 with the Culture of HEK Cells Synchronously Infected with the Virus."}]},"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1976-01-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"158","bibliographicPageStart":"151","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"熱帯医学 Tropical medicine"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"適宜に階段稀釈した抗血清とX×10^4 PFUのウイルスを混じ室温1時間放置した後,直ちに1:100まで稀釈する.この稀釈液と等量の維持に浮游させた2～3×10^6/mlのHEK細胞とを混和し,37℃30分間軽く振盪する.径5cmのシヤレーに1mlずつ入れ,細胞をガラス面に拡げ,0.5%仔牛血清,0.75%カルボオキシメチールセルローズを含んだ維持液を加え蔽う.5日ないし6日目に細胞をギムザ液で染色し,プラークを算える.以上が抗血清の中和抗体の測定法である.抗血清と抗原(分画その他)を加え充分反応させた後,更らにX×10^4PFUのウイルスを追加し室温60分放置し,以後の手順は上記の方法に従う,以上が抗原による抗血清中の抗体のブロック能を知る方法である.以上の方法を用いて,アデノウイルス5型の抗血清に対するA抗原,C抗原及びP抗原のブロック能を検査した.抗血清が完全にブロックされたのはA抗原によってのみであり, C抗原及びP抗原は抗血清のブロック能を有しないことを知った.","subitem_description_type":"Abstract"},{"subitem_description":"The mixture of antiserum and antigen at each adequate dilution was added the seed virus contained X×10^4 PFU per ml. The mixture was diluted upto the concentration contained 50 to 100 plaques per ml per a dish and 2 ml of the last dilution was made. The last dilution of the mixture was added equal volume of HEK cell suspension contained 2 or 3×10^6 cells per ml and shaked at 37℃ for 20 or 30 minutes. One ml of these mixture of antiserum, antigen and seed virus was plated into a dish, and 4 ml of maintainance medium contained 0.5% calf serum and 0.75% carboxymethylcellulose was added and spread over in a dish. After incubation for 5 or 6 days, the cell sheet was fixed with 10% formaldehyde saline solution and washed and stained with Gimsa solution. The plaques formed in this method were clear and easy to count. The activity of antigen A, C and P of adenovirus type 5 for the blocking antibody against purified adenovirus type 5 was studied with the application of this plaque assay method.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"熱帯医学 Tropical medicine 17(3). p151-158, 1976","subitem_description_type":"Other"}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"104441","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ueda, Yoshiaki"}]},{"nameIdentifiers":[{"nameIdentifier":"104442","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Akashi, Mitsunobu"}]},{"nameIdentifiers":[{"nameIdentifier":"104443","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hayashi, Kaoru"}]}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎大学熱帯医学研究所"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00199644","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855643","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"P00151-P00158"}]},"item_3_text_63":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Institute of Tropical Medicine, Nagasaki University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"上田, 芳秋"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"明石, 光伸"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"林, 薫"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-24"}],"displaytype":"detail","filename":"tm17_03_05_t.pdf","filesize":[{"value":"609.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"tm17_03_05_t.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/24815/files/tm17_03_05_t.pdf"},"version_id":"34a2a2b3-b6ad-46f4-9918-c19aa2fa5e43"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"同時感染したHEK細胞を用いたアデアウイルス5型のプラーク法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"同時感染したHEK細胞を用いたアデアウイルス5型のプラーク法"}]},"item_type_id":"3","owner":"2","path":["1810"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-04-26"},"publish_date":"2006-04-26","publish_status":"0","recid":"24815","relation_version_is_last":true,"title":["同時感染したHEK細胞を用いたアデアウイルス5型のプラーク法"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T00:02:18.279342+00:00"}