{"created":"2023-05-15T16:48:03.005141+00:00","id":24939,"links":{},"metadata":{"_buckets":{"deposit":"65218de3-5775-481e-811b-b98e02cc32da"},"_deposit":{"created_by":2,"id":"24939","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"24939"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00024939","sets":["35:1741:1775:1834"]},"author_link":["105172","105175","105173","105174","105177","105176"],"item_3_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Dirofilaria immitis : Physicochemical Properties of IgG-inducing Antigen with Special Reference to the Comparison with Highly Purified Allergen"}]},"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1981-12-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"204","bibliographicPageStart":"193","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"熱帯医学 Tropical medicine"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"われわれは,すでに犬糸状虫成虫体よりアレルゲンを単一の糖蛋白として精製し,その物理・化学的特徴について報告した.今回,宿主にIgG抗体を誘導する物質を犬糸状虫体より分離・精製し,精製アレルゲンの免疫学的および物理化学的諸性質と比較検討した.犬糸状虫由来のIgG誘導抗原は虫体内に多種類存在したが,そのうち最も効果的に宿主にIgG抗体を誘導する物質は,分子量約60万,糖約20%を有する物質で,熱・酸・アルカリおよび振盪などに対して,またトリプシンやキモトリプシン,過ヨード酸や核酸処理などの物理化学的処理に対して極めて強い抵抗性を示した.また,この物質は特別な酵素活性をもたなかった.抗原活性部位は,アレルゲンと同様蛋白部分にあった.この抗原を種々の濃度でラットおよび8系統のマウスに免疫すると,IgG抗体のみ誘導し,IgE抗体の産生は見なかった.なお,この抗原はまだ,2種類の蛋白の混成であることがディスク電気泳動で明らかにされた.この抗原で誘導された抗体は主としてIgG抗体であることが,2-ME処理後の免疫血清,Sephadex G-200ゲル濾過の第2ピークおよびゾーン電気泳動後のDEAE-Cellulose分画にそれぞれ活性が集中する事実によって確認された.","subitem_description_type":"Abstract"},{"subitem_description":"A soluble somatic preparation of adult Dirofilaria immitis was prepared and was found to contain potent antigens for the production of IgG antibody in rats as well as various strains of outbred or inbred mice without any detectable IgE antibody, in spite of the use of aluminum hydroxide as adjuvant. The IgG-inducing antigen was purified in relatively pure form from the soluble somatic preparation, and its physicochemical properties were studied and compared with those of the highly purified allergen. Both the IgG-inducing antigen and the allergen were remarkably stable to various kinds of proteases, periodate digestion and other physicochemical treatments, such as heat, vibration, acid and alkali, but very sensitive to pronase digestion. They had almost no significant enzymatic activities. In contrast to the allergen, IgG-inducing antigen had a larger molecular weight (ca, 600,000) and a higher content of carbohydrate (20%).","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"熱帯医学 Tropical medicine 23(4). p193-204, 1981","subitem_description_type":"Other"}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"105175","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Fujita, Koichiro"}]},{"nameIdentifiers":[{"nameIdentifier":"105176","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tsukidate, Setsuko"}]},{"nameIdentifiers":[{"nameIdentifier":"105177","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ikeda, Teruaki"}]}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎大学熱帯医学研究所"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00199644","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855643","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"P00193-P00204"}]},"item_3_text_63":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Institute of Tropical Medicine, Nagasaki University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤田, 紘一郎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"月館, 説子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"池田, 照明"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-24"}],"displaytype":"detail","filename":"tm23_04_03_t.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"tm23_04_03_t.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/24939/files/tm23_04_03_t.pdf"},"version_id":"2f8d3fa7-df35-4628-a728-154a3c6612ba"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"犬糸状虫由来IgG誘導抗原の精製とその物理・化学的特徴","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"犬糸状虫由来IgG誘導抗原の精製とその物理・化学的特徴"}]},"item_type_id":"3","owner":"2","path":["1834"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-04-26"},"publish_date":"2006-04-26","publish_status":"0","recid":"24939","relation_version_is_last":true,"title":["犬糸状虫由来IgG誘導抗原の精製とその物理・化学的特徴"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-15T23:56:55.588019+00:00"}