{"created":"2023-05-15T16:48:03.607696+00:00","id":24953,"links":{},"metadata":{"_buckets":{"deposit":"e255bfb1-9ab3-4a3e-91ef-cba4950c8674"},"_deposit":{"created_by":2,"id":"24953","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"24953"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00024953","sets":["35:1741:1775:1836"]},"author_link":["105261","105256","105260","105257","105259","105258"],"item_3_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Enzyme-linked Immunosorbent Assay (ELISA) on Japanese Encephalitis Virus. III. Assay on Antibody Titers in Swine Sera"}]},"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1982-06-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"102","bibliographicPageStart":"87","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"熱帯医学 Tropical medicine"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"1681年の長崎県下のと場ブタ血清330検体を用い,日本脳炎(JE)ウイルスに対する抗体価を,免疫酵素抗体法(ELSA)の間接法により測定した. ELISAによる抗JE免疫グロブリン抗体価の検出と血球凝集抑制法(HI)によるものは,ほとんど一致した.抗JE抗体のIgMクラスは, 2メルカプトエタノール(2ME)処理によるHI法で, 33検体が検出されたのに比べて, ELISAでは97検体が検出された. 97検体中, 65検体はELISAでのみIgM抗体が認められたが,一方, 2ME感受性HI抗体が証明され, IgM-ELISA抗体が陰性のものが1検体あった. IgM抗体陽性で2-ME感受性HI抗体が陰性の検体の大部分は,流行の末期の高い抗体価の認められたものであった.","subitem_description_type":"Abstract"},{"subitem_description":"Antibody titers against Japanese encephalitis (JE) virus were measured by indirect enzyme-linked immunosorbent assay (ELISA) using 330 sera from slaughtered swines in Nagasaki, 1981. Detection of anti-JE immunoglobulin (Ig) antibodies by the ELISA almost paralleled to that by the hemagglutination-inhibition (HI) and the titer by the ELISA was approximately 10 times higher than that by the HI. Presence of IgM class of anti-JE antibodies was detected in 97 specimens by the ELISA in contrast to 33 by the HI test using 2-mercaptoethanol (2ME) treatment. Six+y five of the 97 specimens were shown to possess IgM antibodies only by the ELISA, while there was a single specimen with demonstrable 2ME-sensitive HI antibodies and negative IgM-ELISA. Most of the specimens with positive IgM-ELISA and negative 2ME-sensitive HI antibodies were those with high-titered Ig antibodies found late in the epidemic season.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"熱帯医学 Tropical medicine 24(2). p87-102, 1982","subitem_description_type":"Other"}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"105259","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Bundo, Keiko"}]},{"nameIdentifiers":[{"nameIdentifier":"105260","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Morita, Koichi"}]},{"nameIdentifiers":[{"nameIdentifier":"105261","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Igarashi, Akira"}]}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎大学熱帯医学研究所"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00199644","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855643","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"P00087-P00102"}]},"item_3_text_63":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Institute of Tropical Medicine, Nagasaki University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"分藤, 桂子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"森田, 公一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"五十嵐, 章"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-24"}],"displaytype":"detail","filename":"tm24_02_05_t.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"tm24_02_05_t.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/24953/files/tm24_02_05_t.pdf"},"version_id":"7d615922-4e31-4c3b-ad0e-1cf646b7dca5"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"日本脳炎ウイルスに対する免疲酵素測定法(ELISA). III.ブタ血清中の抗体価の測定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"日本脳炎ウイルスに対する免疲酵素測定法(ELISA). III.ブタ血清中の抗体価の測定"}]},"item_type_id":"3","owner":"2","path":["1836"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-04-26"},"publish_date":"2006-04-26","publish_status":"0","recid":"24953","relation_version_is_last":true,"title":["日本脳炎ウイルスに対する免疲酵素測定法(ELISA). III.ブタ血清中の抗体価の測定"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-15T23:56:15.103003+00:00"}