@article{oai:nagasaki-u.repo.nii.ac.jp:00025077,
 author = {五十嵐, 章 and 松尾, 幸子 and 分藤(森田), 桂子 and 小田, 力 and 森, 章夫 and 藤田, 絋一郎},
 issue = {3},
 journal = {熱帯医学 Tropical medicine},
 month = {Sep},
 note = {蚊の昆虫ウイルスの一つを数種類の実験室内累代継代蚊の幼虫に感染させた時の殺虫効果を調べた.殺虫効果はウイルスの濃度,蚊の種類,及び幼虫の成熟度によって異なった.四令幼虫にウイルスを感染させた場合,累積死亡率はチカイエカの13%からシマハマダラカの72%であったが対照群の死亡率は0-8%であった.ネッタイシマカの場合,四令幼虫にウイルスを感染させた時最も高い死亡率を示し若令幼虫に感染された時の死亡率は低かった.この傾向はコガタアカイエカ及びシナハマダラカでも認められたが,ヒトスジシマカとチカイエカの場合には逆の傾向が見られ,第一令幼虫に感染された方が三又は四令幼虫に感染させた時よりも高い死亡率を示した.死亡した幼虫は脱水し縮小した外観を呈したが,ウイルス増殖は死亡した幼虫だけでなく一見健康に見える幼虫にも認められた.ウイルス感染幼虫から羽化した雌成虫から卵を介して次代の蚊にウイルスが伝達される経卵感染は証明されなかった.この昆虫ウイルス(ヨコショウジウイルス)をマウス脳内に接種しても病的変化は認められなかった.長崎県下のブタとウシの抗体調査ではヨコショウジウイルスの自然感染を示す結果は得られなかった., Effect of a mosquito insect virus infection on several laboratory-colonized mosquito larvae were investigated. Larvicidal effect was dependent on the virus dose, mosquito species and also the stage of the larvae. When 4th instar larvae were infected, cumulative mortality of mosquitoes were from 13 to 72% (highest with Anopheles sinensis and lowest with Culex pipiens molestus), in contrast to 0-8% in the control (highest with Cx. p. molestus and lowest with Aedes albopictus ). In the case of Ae. aegypti, cumulative mortality was highest when the virus was infected to the 4th instar larvae and the less mortality was recorded when earlier instar larvae were infected. This tendency was also observed for Cx. tritaeniorhynchus and An. sinensis. However, opposite tendency was observed for Ae, albopictus, and Cx. p. molestus with higher mortality when the lst instar larvae were infected than the 4th or 3rd instar. The higher mortality resulted in the lower pupation or emergence rate. The dead larvae appeared to have shrunk and dehydrated. Virus growth was observed not only in dead larvae but also in apparently healthy ones. No transovarial transmission of the virus was observed from the adult females emerged from virus-infected larvae to the next generation. No pathological effects or appreciable antibody production were observed by intracerebral inoculation of Yokoshoji virus to weaning mice. Antibody survey among swine and bovine population in Nagasaki Prefecture did not reveal any indication of Yokoshoji virus infection in nature., 熱帯医学 Tropical medicine 28(3). p191-207, 1986},
 pages = {191--207},
 title = {昆虫ウイルスの蚊幼虫に対する殺虫効果 : 蚊の生物学的防除としての可能性について},
 volume = {28},
 year = {1986}
}