@article{oai:nagasaki-u.repo.nii.ac.jp:00025171,
 author = {Shameem, Golam Masud Mohammad and Morita, Kouichi and Tanaka, Mariko and Igarashi, Akira},
 issue = {2},
 journal = {熱帯医学 Tropical medicine},
 month = {Jun},
 note = {Two Japanese encephalitis (JE) virus strains, namely a virulent JaOH0556 and an attenuated ML-17 derived from it were studied to investigate their in vitro phenotypic differences. When both strains were concentrated by polyethylene glycol (PEG) 6000 precipitation, infectivity of the ML-17 was recovered only 4.94% compared to 42.21% for the parental JaOH0566 strain, and the virion peak was very low for the ML-17 in contrast to a sharp peak for the JaOH0566 in sucrose density gradient centrifugation. When both strains were incubated with sucrose solutions, the ML-17 was slightly more labile than the JaOH0566 at high concentration of sucrose. These findings may indicate difference in the virion structure of both strains., 熱帯医学 Tropical medicine 31(2). p67-74, 1989},
 pages = {67--74},
 title = {In Vitro Difference between an Attenuated ML-17 and its Parental Virulent JaOH0566 Strain of Japanese Encephalitis Virus},
 volume = {31},
 year = {1989}
}