{"created":"2023-05-15T16:48:13.574951+00:00","id":25181,"links":{},"metadata":{"_buckets":{"deposit":"337b7d3d-fdd1-4e3e-bb1d-205e99387f8b"},"_deposit":{"created_by":2,"id":"25181","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"25181"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00025181","sets":["35:1741:1775:1867"]},"author_link":["106987","106986","106988","106985"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1989-12-27","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"159","bibliographicPageStart":"151","bibliographicVolumeNumber":"31","bibliographic_titles":[{"bibliographic_title":"熱帯医学 Tropical medicine"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Optimal conditions for the infectivity titration by plaque formation (PFU) of Japanese encephalitis (JE) virus on mammalian BHK21 cells were investigated. Early virus inoculation at 6 hr after seeding low concentration of the cells (0.25×10^5/ml) produced larger and clearer plaques compared with higher concentration or longer incubation (1-2 days) of the cells. However, the virus titer increased by the incubation time until 2 days to become the highest for this inoculum cell concentration, and up to 1 day for the inoculum cell concentration of 0.5 or 1.0×10^5/ml. While the virus titer did not differ significantly by the time of cell culture for the inoculum cell concentration of 2.0×10^5/ml. Supplementation of 9% fetal bovine serum (FBS) to the cell growth medium was mandatory for clear plaque formation. Among several methyl cellulose (MC) concentrations in the overlay medium, a moderate concentration of 1.25% was optimal to produce clear and distinct plaques on 1 day's culture from 2.0×10^5 cells/ml.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"熱帯医学 Tropical medicine 31(4). p151-159, 1989","subitem_description_type":"Other"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎大学熱帯医学研究所"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00199644","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855643","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"P00151-P00159"}]},"item_3_text_63":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Institute of Tropical Medicine, Nagasaki University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shameem, Golam Masud Mohammad"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Kouichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Haishi, Shozo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Igarashi, Akira"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-24"}],"displaytype":"detail","filename":"tm31_04_01_t.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"tm31_04_01_t.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/25181/files/tm31_04_01_t.pdf"},"version_id":"d1b27a52-8bac-42c4-b07d-c06c42be3277"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Optimal Conditions of Plaque Titration of Japanese Encephalitis Virus on BHK21 Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Optimal Conditions of Plaque Titration of Japanese Encephalitis Virus on BHK21 Cells"}]},"item_type_id":"3","owner":"2","path":["1867"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-04-26"},"publish_date":"2006-04-26","publish_status":"0","recid":"25181","relation_version_is_last":true,"title":["Optimal Conditions of Plaque Titration of Japanese Encephalitis Virus on BHK21 Cells"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-15T23:46:43.079081+00:00"}