@article{oai:nagasaki-u.repo.nii.ac.jp:00025195,
 author = {Srivastava, Ashok Kumar and Morita, Kouichi and Igarashi, Akira},
 issue = {3},
 journal = {熱帯医学 Tropical medicine},
 month = {Sep},
 note = {Envelope glycoprotein (E) of Japanese encephalitis (JE) virus was prepared from purified virion by Triton X-100 (TX-100) treatment (E-TX), polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulphate (SDS) after reduction (E-2ME-SDS) or without reduction (E-IAA-SDS), and high performance liquid chromatography after denaturation with guanidine hydrochloride (E-HPLC). E-2ME-SDS, E-IAA-SDS and particularly E-HPLC were less immunogenic than E-TX, complete virion or formalin-inactivated JE vaccine, which were almost similarly immunogenic to produce anti-JE ELISA and neutralizing (N) antibodies. E-HPLC produced low but definite N antibodies with highest N/ELISA ratio among all immunogens tested. The result indicated that most of the JE virus E protein N and ELISA epitopes were conformational, but some N epitope(s) were resistant to denaturation and could be preserved in E-HPLC better than the ELISA epitopes., 熱帯医学 Tropical medicine 32(3). p103-113, 1990},
 pages = {103--113},
 title = {Immunogenicity of Japanese Encephalitis Virus Envelope Glycoprotein E Prepared by Four Different Methods},
 volume = {32},
 year = {1990}
}