{"created":"2023-05-15T16:48:14.210953+00:00","id":25195,"links":{},"metadata":{"_buckets":{"deposit":"78c78e75-c5ad-4629-af82-902f8597364e"},"_deposit":{"created_by":2,"id":"25195","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"25195"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00025195","sets":["35:1741:1775:1870"]},"author_link":["107032","107033","107031"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1990-09-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"113","bibliographicPageStart":"103","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"熱帯医学 Tropical medicine"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Envelope glycoprotein (E) of Japanese encephalitis (JE) virus was prepared from purified virion by Triton X-100 (TX-100) treatment (E-TX), polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulphate (SDS) after reduction (E-2ME-SDS) or without reduction (E-IAA-SDS), and high performance liquid chromatography after denaturation with guanidine hydrochloride (E-HPLC). E-2ME-SDS, E-IAA-SDS and particularly E-HPLC were less immunogenic than E-TX, complete virion or formalin-inactivated JE vaccine, which were almost similarly immunogenic to produce anti-JE ELISA and neutralizing (N) antibodies. E-HPLC produced low but definite N antibodies with highest N/ELISA ratio among all immunogens tested. The result indicated that most of the JE virus E protein N and ELISA epitopes were conformational, but some N epitope(s) were resistant to denaturation and could be preserved in E-HPLC better than the ELISA epitopes.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"熱帯医学 Tropical medicine 32(3). p103-113, 1990","subitem_description_type":"Other"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎大学熱帯医学研究所"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00199644","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855643","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"P00103-P00113"}]},"item_3_text_63":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Institute of Tropical Medicine, Nagasaki University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Srivastava, Ashok Kumar"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Kouichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Igarashi, Akira"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-24"}],"displaytype":"detail","filename":"tm32_03_02_t.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"tm32_03_02_t.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/25195/files/tm32_03_02_t.pdf"},"version_id":"dd2a0129-d50d-4a04-b98a-d89c0ffa67b3"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Immunogenicity of Japanese Encephalitis Virus Envelope Glycoprotein E Prepared by Four Different Methods","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Immunogenicity of Japanese Encephalitis Virus Envelope Glycoprotein E Prepared by Four Different Methods"}]},"item_type_id":"3","owner":"2","path":["1870"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-04-26"},"publish_date":"2006-04-26","publish_status":"0","recid":"25195","relation_version_is_last":true,"title":["Immunogenicity of Japanese Encephalitis Virus Envelope Glycoprotein E Prepared by Four Different Methods"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-15T23:46:03.170821+00:00"}