{"created":"2023-05-15T16:48:15.023639+00:00","id":25211,"links":{},"metadata":{"_buckets":{"deposit":"aba0f9bf-6788-4bcd-a729-d2ff2d8da84e"},"_deposit":{"created_by":2,"id":"25211","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"25211"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00025211","sets":["35:1741:1775:1873"]},"author_link":["107095","107094","107096","107097","107093"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-09-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"82","bibliographicPageStart":"67","bibliographicVolumeNumber":"33","bibliographic_titles":[{"bibliographic_title":"熱帯医学 Tropical medicine"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A DNA-Binding protein was purified from the cell-surface of Vibrio cholerae O1 by Cellulofine Sulfate affinity chromatography and reversed-phase HPLC. The DNA-binding protein of V. cholerae O1 showed a molecular weight of 9 kDa estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Treatment of the protein with cold acetone and 6 M guanidine-HCl produced little change in the DNA-binding activity of the protein. The binding of the protein to supercoiled plasmid DNA as well as to double-stranded short chromosomal DNA fragments has been demonstrated by gel retardation assay and by labeling of the transblotted protein with non-radioactive DNA. N-terminal amino acid sequence of the protein showed a high degree of homology with that of HU 2 (Laine et al., 1978), NS2 (Mende et al., 1978) from Escherichia coli. Indirect immunocytochemical labeling of ultrathin sections of Lowicryl-embedded V. cholerae O1 cells with the antibody against the protein revealed that the protein associates with bulk DNA and also lies at the cell-surface.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"熱帯医学 Tropical medicine 33(3). p67-82, 1991","subitem_description_type":"Other"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎大学熱帯医学研究所"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00199644","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855643","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"P00067-P00082"}]},"item_3_text_63":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Institute of Tropical Medicine, Nagasaki University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ehara, Masahiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ichinose, Yoshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwami, Mamoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Utsunomiya, Akiyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimotori, Shoichi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-24"}],"displaytype":"detail","filename":"tm33_03_05_t.pdf","filesize":[{"value":"3.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"tm33_03_05_t.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/25211/files/tm33_03_05_t.pdf"},"version_id":"8b51ba57-8110-456b-8f52-213af4f9bb40"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification and Characterization of a DNA-Binding Protein of Vibrio cholerae O1","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification and Characterization of a DNA-Binding Protein of Vibrio cholerae O1"}]},"item_type_id":"3","owner":"2","path":["1873"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-04-26"},"publish_date":"2006-04-26","publish_status":"0","recid":"25211","relation_version_is_last":true,"title":["Purification and Characterization of a DNA-Binding Protein of Vibrio cholerae O1"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-15T23:45:22.236331+00:00"}