@article{oai:nagasaki-u.repo.nii.ac.jp:00025256,
 author = {Suenaga, Osamu},
 issue = {3},
 journal = {熱帯医学 Tropical medicine},
 month = {Dec},
 note = {A wild type strain of Culex pipiens molestus, which was originated from one egg raft collected by an ovi-trap in Nagasaki, Japan in 1980 and maintained at an insectarium of 25℃ and 70% RH, was cured by exposing the first instar larvae to 2.5-5%, 5%, 10%, and 20% water solutions of tetracycline hydrochloride for 20-48 hours befor food was added. Only one out of 12 lines of the strain has been cured of the Wolbachia infection when it was treated by 5% solution for 24 hours. Although treated and aposymbiotic (or Wolbachia free) females produced viable progeny when they mated with aposymbiotic males, no progeny was produced by the females when they were backcrossed with the original males with symbiotes. The newly established aposymbiotic strain has been maintained for over 11 generations., 熱帯医学 Tropical medicine 35(3). p105-110, 1993},
 pages = {105--110},
 title = {Treatment of Wolbachia pipientis Infection with Tetracycline Hydrochloride and the Change of Cytoplasmic Incompatibility in a Nagasaki Strain of Culex pipiens molestus},
 volume = {35},
 year = {1993}
}