{"created":"2023-05-15T16:48:20.056940+00:00","id":25328,"links":{},"metadata":{"_buckets":{"deposit":"cea132bd-895e-4515-a89c-14ac3f3acfbc"},"_deposit":{"created_by":2,"id":"25328","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"25328"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00025328","sets":["35:1741:1775:1887"]},"author_link":["107469","107471","107470","107472","107473","107474"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-06-23","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"33","bibliographicPageStart":"29","bibliographicVolumeNumber":"37","bibliographic_titles":[{"bibliographic_title":"熱帯医学 Tropical medicine"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"This study was carried out to compare the efficiency of two different purification methods used for removing excess primers and short oligonucleotides from PCR products. Our aim was to sequence PCR product directly. The two different purification methods were micro filter spin unit system (Millipore) and Quick Spin Column (Boehringer Mannheim Biochemica). The results indicated that the purification method using microfilter spin unit system was better and yielded better suquencing pattern compared to quick Spin Column.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"熱帯医学 Tropical medicine 37(1). p29-33, 1995","subitem_description_type":"Other"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎大学熱帯医学研究所"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00199644","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855643","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"P00029-P00033"}]},"item_3_text_63":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Institute of Tropical Medicine, Nagasaki University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Thayan, Ravindran"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Kouichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Vijayamalar, Balasubramaniam"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chew, Tong Keong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sinniah, Mangalam"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Igarashi, Akira"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-24"}],"displaytype":"detail","filename":"tm37_01_04_t.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"tm37_01_04_t.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/25328/files/tm37_01_04_t.pdf"},"version_id":"40c712e8-4a48-4ee9-9104-9d0f02d99320"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Comparison of Two Different Methods for the Purification of Polymerase Chain Reaction (PCR) Products Used in Direct Sequencing, in an Applied Biosystems 373A DNA Sequencing System","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Comparison of Two Different Methods for the Purification of Polymerase Chain Reaction (PCR) Products Used in Direct Sequencing, in an Applied Biosystems 373A DNA Sequencing System"}]},"item_type_id":"3","owner":"2","path":["1887"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-04-26"},"publish_date":"2006-04-26","publish_status":"0","recid":"25328","relation_version_is_last":true,"title":["Comparison of Two Different Methods for the Purification of Polymerase Chain Reaction (PCR) Products Used in Direct Sequencing, in an Applied Biosystems 373A DNA Sequencing System"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-15T23:40:17.395008+00:00"}