{"created":"2023-05-15T16:48:20.471377+00:00","id":25337,"links":{},"metadata":{"_buckets":{"deposit":"34e1ede9-b070-4ae3-8f14-06ea0b0ed4e2"},"_deposit":{"created_by":2,"id":"25337","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"25337"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00025337","sets":["35:1741:1775:1889"]},"author_link":["107514","107510","107513","107515","107512","107511"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-12-26","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"114","bibliographicPageStart":"109","bibliographicVolumeNumber":"37","bibliographic_titles":[{"bibliographic_title":"熱帯医学 Tropical medicine"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In order to distribute dengue IgM-capture ELISA to the peripheral laboratories, supply of key reagents should be established by local production. In this study, comparative IgM-capture ELISA was carried out on a total of 237 sera using key reagents from 2 sources. The current set consisted of suckling mouse brain (SMB)-derived dengue type 2 (D2) antigen and a D2 monoclonal antibody (MAb) supplied from the United States. While an alternative set was supplied by one of the authors, consisting of tissue culture-derived dengue antigen (TCA) and MAb established in USM. When the IgM-ELISA results were compared with those by the hemagglutination-inhibition (HI) test as a gold standard, the sensitivity was 50.0% by the current set and 75.0% by the alternative set of reagents, respectively. While the specificity was 95.2% by the current set and 90.3% by the alternative set of reagents. The results showed that the alternative set of reagents can effectively be used in dengue IgM-ELISA and is significantly more sensitive than the current set of reagents (Chi SQR=12.26, P<0.01) at less than 1% risk. However there is no significant difference in specificity between the two reagents (Chi SQR=2.45, 0.5>P>0.1) at 10% risk.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"熱帯医学 Tropical medicine 37(3). p109-114, 1995","subitem_description_type":"Other"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎大学熱帯医学研究所"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00199644","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855643","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"P00109-P00114"}]},"item_3_text_63":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Institute of Tropical Medicine, Nagasaki University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mohamed, Halimah"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Balasubramaniam, Vijayamalar"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Cardosa, Jane"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sinniah, Mangalam"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Igarashi, Akira"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Hiroshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-24"}],"displaytype":"detail","filename":"tm37_03_03_t.pdf","filesize":[{"value":"467.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"tm37_03_03_t.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/25337/files/tm37_03_03_t.pdf"},"version_id":"bc4446e0-afb5-4291-86a0-629c6acca1df"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Comparative Assay on Dengue IgM-ELISA Using Reagents From 2 Sources","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Comparative Assay on Dengue IgM-ELISA Using Reagents From 2 Sources"}]},"item_type_id":"3","owner":"2","path":["1889"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-04-26"},"publish_date":"2006-04-26","publish_status":"0","recid":"25337","relation_version_is_last":true,"title":["Comparative Assay on Dengue IgM-ELISA Using Reagents From 2 Sources"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-15T23:39:56.151974+00:00"}