@article{oai:nagasaki-u.repo.nii.ac.jp:00025344,
 author = {Igarashi, Akira and Mohamed, Haliman and Yusof, Apandi and Sinniah, Mangalam and Tanaka, Hiroshi},
 issue = {4},
 journal = {熱帯医学 Tropical medicine},
 month = {Mar},
 note = {In order to strengthen laboratory diagnostic capability on dengue by IgM-ELISA in Malaysia, type 2 dengue (D2) monoclonal antibody (MAb) and cell culture-derived D2 antigen were prepared as culture fluid of hybridoma cell line and D2-infected C6/36 culture fluid, respectively. Comparative IgM-ELISA was carried out on 90 serum specimens from dengue patients using newly prepared reagents in comparison with routine reagents (D2-MAb mouse ascitic fluid from CDC, USA, and D2 antigen extracted from infected suckling mouse brains). The newly prepared MAb at 2-10 fold higher concentration than that estimated by antigen detection ELISA, in combination with the undiluted D2-infected C6/36 cell culture fluid, provided comparable sensitivity (93.3-97.9%) to that of routine reagents (90.0-98.5%)., 熱帯医学 Tropical medicine 37(4). p165-173, 1996},
 pages = {165--173},
 title = {Production of Type 2 Dengue (D2) Monoclonal Antibody and Cell Culture Derived D2 Antigen for Use in Dengue IgM-Capture ELISA},
 volume = {37},
 year = {1996}
}