{"created":"2023-05-15T16:31:12.337315+00:00","id":2563,"links":{},"metadata":{"_buckets":{"deposit":"ac3c96ad-8a2b-4b81-bf01-5d9fc902dbd8"},"_deposit":{"created_by":2,"id":"2563","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"2563"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00002563","sets":["35:36"]},"author_link":["11937","11942","11935","11943","11936","11940","11939","11938","11941"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-11","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"110","bibliographicPageStart":"102","bibliographicVolumeNumber":"249","bibliographic_titles":[{"bibliographic_title":"Journal of Virological Methods"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"This study describes the first multiway comparison of portable isothermal assays for the detection of foot-and-mouth disease virus (FMDV), benchmarked against real-time reverse transcription RT-PCR (rRT-PCR). The selected isothermal chemistries included reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription recombinase polymerase amplification (RT-RPA). The analytical sensitivity of RT-LAMP was comparable to rRT-PCR (101 RNA copies), while RT-RPA was one log10 less sensitive (102 RNA copies). Diagnostic performance was evaluated using a panel of 35 samples from FMDV-positive cattle and eight samples from cattle infected with other vesicular viruses. Assay concordance for RT-LAMP and RT-RPA was 86?98% and 67?77%, respectively, when compared to rRT-PCR, with discordant samples consistently having high rRT-PCR cycle threshold values (no false-positives were detected for any assay). In addition, a hierarchy of sample preparation methods, from robotic extraction to simple dilution of samples, for epithelial suspensions, serum and oesophageal-pharyngeal (OP) fluid were evaluated. Results obtained for RT-LAMP confirmed that FMDV RNA can be detected in the absence of RNA extraction. However, simple sample preparation methods were less encouraging for RT-RPA, with accurate results only obtained when using RNA extraction. Although the evaluation of assay performance is specific to the conditions tested in this study, the compatibility of RT-LAMP chemistry with multiple sample types, both in the presence and absence of nucleic acid extraction, provides advantages over alternative isothermal chemistries and alternative pen-side diagnostics such as antigen-detection lateral-flow devices. These characteristics of RT-LAMP enable the assay to be performed over a large diagnostic detection window, providing a realistic means to rapidly confirm positive FMD cases close to the point of sampling.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Virological Methods, 249, pp.102-110; 2017","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jviromet.2017.08.013","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/)."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01660934","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Howson, Emma L.A."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kurosaki, Yohei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yasuda, Jiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Masayoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Goto, Hiroaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Gray, Ashley R."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mioulet, Valerie"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"King, Donald P."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fowler, Veronica L."}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"JVM249_102.pdf","filesize":[{"value":"941.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JVM249_102.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/2563/files/JVM249_102.pdf"},"version_id":"caee4da0-f0e1-4685-b87f-ebee4afe3518"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Foot-and-Mouth disease","subitem_subject_scheme":"Other"},{"subitem_subject":"FMDV","subitem_subject_scheme":"Other"},{"subitem_subject":"Diagnostics","subitem_subject_scheme":"Other"},{"subitem_subject":"rRT-PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"RT-LAMP","subitem_subject_scheme":"Other"},{"subitem_subject":"RT-RPA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Defining the relative performance of isothermal assays that can be used for rapid and sensitive detection of foot-and-mouth disease virus","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Defining the relative performance of isothermal assays that can be used for rapid and sensitive detection of foot-and-mouth disease virus"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-09-26"},"publish_date":"2017-09-26","publish_status":"0","recid":"2563","relation_version_is_last":true,"title":["Defining the relative performance of isothermal assays that can be used for rapid and sensitive detection of foot-and-mouth disease virus"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:44:18.002891+00:00"}