{"created":"2023-05-15T16:29:29.660351+00:00","id":262,"links":{},"metadata":{"_buckets":{"deposit":"91245276-0caf-473c-b6f2-d003ade15651"},"_deposit":{"created_by":2,"id":"262","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"262"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000262","sets":["10:11"]},"author_link":["1312","1310","1314","1311","1313"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-08-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"774","bibliographicPageStart":"772","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"Chemical and Pharmaceutical Bulletin"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A series of chemiluminescent compounds containing a hydrazide group as a nucleophilic functional group has been synthesized. The syntheses were started from chemiluminescent luminol and isoluminol. The linker moiety was easily introduced onto non-nucleophilic exocyclic amino groups of luminol and isoluminol by gentle heating with cyclic acid anhydrides such as glutaric anhydride. The resulting carboxy group was converted to hydrazide by a simple condensation reaction using carbodiimide. Although majority of the synthesized compounds did not emit strong light, a sufficient chemiluminescence intensity was obtained from luminolamido- C2-hydrazide (L2H) comprising of luminol scaffold with a dimethylene linker. The ability of L2H to form a covalent bond with a macromolecule was further investigated by incubation with oxidized horseradish peroxidase. The analysis on matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) MS revealed that the coupling efficiency of L2H was similar to that of commercially available labelling reagent having a hydrazide group. These results suggested that L2H, the luminol hydrazide containing a dimethylene linker, could be useful for the labelling of macromolecules in the sensitive bioassay such as chemiluminescence immunoassay.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Chemical and Pharmaceutical Bulletin, 67(8), pp.772-774; 2019","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本薬学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1248/cpb.c19-00126","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2019 The Pharmaceutical Society of Japan."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00092363","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13475223","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Pharmaceutical Society of Japan"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shibata, Takayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshimura, Hiroki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamayoshi, Asako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuda, Nobuaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Dragusha, Shpend"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"CPBul67_772.pdf","filesize":[{"value":"419.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CPBul67_772.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/262/files/CPBul67_772.pdf"},"version_id":"2403f5c0-7814-4968-bf75-61af103596ad"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chemiluminescence","subitem_subject_scheme":"Other"},{"subitem_subject":"Hydrazide","subitem_subject_scheme":"Other"},{"subitem_subject":"Labelling reagent","subitem_subject_scheme":"Other"},{"subitem_subject":"Luminol","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Hydrazide Derivatives of Luminol for Chemiluminescence-Labelling of Macromolecules","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hydrazide Derivatives of Luminol for Chemiluminescence-Labelling of Macromolecules"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-08-29"},"publish_date":"2019-08-29","publish_status":"0","recid":"262","relation_version_is_last":true,"title":["Hydrazide Derivatives of Luminol for Chemiluminescence-Labelling of Macromolecules"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:19:09.294525+00:00"}