@article{oai:nagasaki-u.repo.nii.ac.jp:00026204, author = {AIBARA, Nozomi and AIZAWA, Rika and NAKASHIMA, Mikiro and OHYAMA, Kaname}, issue = {11}, journal = {Analytical Sciences}, month = {Nov}, note = {The identification of antigens incorporated into immune complexes (IC-antigens) is important for studying the pathophysiology of immunological diseases. Immune complexome analysis identifies IC-antigens by analyzing ICs collected from biological fluids by IC-capturing beads. In this study, we optimized the method to improve its comprehensiveness while maintaining selectivity for IC-antigens by comparing the number of identified peptides (model IC experiment) or proteins (human pooled serum) eluted from Protein G beads using different pH solutions (pH 2.0 - 11.0)., Analytical sciences 36(11), pp.1423-1426; 2020}, pages = {1423--1426}, title = {Optimization of pH Elution Conditions in Immune Complexome Analysis for Comprehensive Identification of Immune Complex Antigens}, volume = {36}, year = {2020} }