@article{oai:nagasaki-u.repo.nii.ac.jp:00026221,
 author = {Yoshikawa, Rokusuke and Abe, Haruka and Igasaki, Yui and Negishi, Saeki and Goto, Hiroaki and Yasuda, Jiro},
 issue = {11},
 journal = {PLOS Neglected Tropical Diseases},
 month = {Nov},
 note = {Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly pathogenic novel coronavirus that has caused a worldwide outbreak.Here we describe a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that uses a portable device for efficient 
detection of SARS-CoV-2. This RT-LAMP assay specifically detected SARS-CoV-2 without cross-reacting with the most closely related human coronavirus, SARS-CoV. Clinical evaluation of nasal swab samples from suspected SARS-CoV-2 pneumonia (COVID-19) patients showed that the assay could detect over 23.7 copies within 15 min with a 100% probability. Since the RT-LAMP assay can be performed with a portable battery-supported device, it is a rapid, simple, and sensitive diagnostic assay for COVID-19 that can be available at point-of-care. We also developed the RT-LAMP assay without the RNA extraction step-Direct RT-LAMP, which could detect more than 1.43 x 103 copies 
within 15 min with a 100% probability in clinical evaluation test. Although the Direct RT-LAMP assay was less sensitive than 
the standard RT-LAMP, the Direct RT-LAMP assay can be available as the rapid first screening of COVID-19 in poorly equipped areas, such as rural areas in developing countries., PLoS neglected tropical diseases, 14(11), art.no.e0008855; 2020},
 title = {Development and evaluation of a rapid and simple diagnostic assay for COVID-19 based on loop-mediated isothermal amplification},
 volume = {14},
 year = {2020}
}