{"created":"2023-05-15T16:48:58.712242+00:00","id":26221,"links":{},"metadata":{"_buckets":{"deposit":"8c3ed610-529d-4869-a3ba-93044a031c64"},"_deposit":{"created_by":2,"id":"26221","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"26221"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026221","sets":["35:36"]},"author_link":["111859","111863","111861","111862","111860","111864"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-11-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageStart":"e0008855","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"PLOS Neglected Tropical Diseases"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly pathogenic novel coronavirus that has caused a worldwide outbreak.Here we describe a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that uses a portable device for efficient \ndetection of SARS-CoV-2. This RT-LAMP assay specifically detected SARS-CoV-2 without cross-reacting with the most closely related human coronavirus, SARS-CoV. Clinical evaluation of nasal swab samples from suspected SARS-CoV-2 pneumonia (COVID-19) patients showed that the assay could detect over 23.7 copies within 15 min with a 100% probability. Since the RT-LAMP assay can be performed with a portable battery-supported device, it is a rapid, simple, and sensitive diagnostic assay for COVID-19 that can be available at point-of-care. We also developed the RT-LAMP assay without the RNA extraction step-Direct RT-LAMP, which could detect more than 1.43 x 103 copies \nwithin 15 min with a 100% probability in clinical evaluation test. Although the Direct RT-LAMP assay was less sensitive than \nthe standard RT-LAMP, the Direct RT-LAMP assay can be available as the rapid first screening of COVID-19 in poorly equipped areas, such as rural areas in developing countries.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLoS neglected tropical diseases, 14(11), art.no.e0008855; 2020","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pntd.0008855","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2020 Yoshikawa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19352735","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yoshikawa, Rokusuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Abe, Haruka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Igasaki, Yui"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Negishi, Saeki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Goto, Hiroaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yasuda, Jiro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-24"}],"displaytype":"detail","filename":"PloSNeg14_8855.pdf","filesize":[{"value":"825.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PloSNeg14_8855.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26221/files/PloSNeg14_8855.pdf"},"version_id":"4a652269-5be3-4221-894e-793cb014973b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development and evaluation of a rapid and simple diagnostic assay for COVID-19 based on loop-mediated isothermal amplification","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development and evaluation of a rapid and simple diagnostic assay for COVID-19 based on loop-mediated isothermal amplification"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-12-04"},"publish_date":"2020-12-04","publish_status":"0","recid":"26221","relation_version_is_last":true,"title":["Development and evaluation of a rapid and simple diagnostic assay for COVID-19 based on loop-mediated isothermal amplification"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-15T23:03:12.258315+00:00"}